Rahmani Nanik, Kahar Prihardi, Lisdiyanti Puspita, Hermiati Euis, Lee Jaemin, Prasetya Bambang, Ogino Chiaki, Kondo Akihiko
a Research Center for Biotechnology , Indonesian Institute of Sciences, Cibinong Science Center , Cibinong , Indonesia.
b Department of Chemical and Engineering, Graduate School of Engineering , Kobe University , Kobe , Japan.
Biosci Biotechnol Biochem. 2018 Feb 23:1-12. doi: 10.1080/09168451.2018.1438169.
The addition of enzymes that are capable of degrading hemicellulose has a potential to reduce the need for commercial enzymes during biomass hydrolysis in the production of fermentable sugars. In this study, a high xylanase producing actinomycete strain (Kitasatospora sp. ID06-480) and the first ethyl ferulate producing actinomycete strain (Nonomuraea sp. ID06-094) were selected from 797 rare actinomycetes, respectively, which were isolated in Indonesia. The addition (30%, v/v) of a crude enzyme supernatant from the selected strains in sugarcane bagasse hydrolysis with low-level loading (1 FPU/g-biomass) of Cellic® CTec2 enhanced both the released amount of glucose and reducing sugars. When the reaction with Ctec2 was combined with crude enzymes containing either xylanase or feruloyl esterase, high conversion yield of glucose from cellulose at 60.5% could be achieved after 72 h-saccharification.
添加能够降解半纤维素的酶有潜力减少在生产可发酵糖的生物质水解过程中对商业酶的需求。在本研究中，分别从797株在印度尼西亚分离得到的稀有放线菌中筛选出一株高产木聚糖酶的放线菌菌株（北里孢菌属ID06 - 480）和第一株产阿魏酸乙酯的放线菌菌株（野野村氏菌属ID06 - 094）。将所选菌株的粗酶上清液以30%（v/v）添加到低水平负载（1 FPU/g生物质）的Cellic® CTec2对甘蔗渣的水解反应中，可提高葡萄糖和还原糖的释放量。当Ctec2反应与含有木聚糖酶或阿魏酸酯酶的粗酶结合时，糖化72小时后，纤维素转化为葡萄糖的产率可高达60.5%。
