Unit Immuno-Microbio Environmental and Carcinogenesis (IMEC), Faculty of Sciences of Bizerte, Carthage University, Jarzouna, Tunisia.
Unit Immuno-Microbio Environmental and Carcinogenesis (IMEC), Faculty of Sciences of Bizerte, Carthage University, Jarzouna, Tunisia.
Kaohsiung J Med Sci. 2018 Mar;34(3):125-133. doi: 10.1016/j.kjms.2017.10.002. Epub 2017 Oct 26.
The aim of the present work was to study the immune profiling of prostate epithelial cells by the expression of ASK-1/p38 and Raf-1/ERK MAP Kinases signaling pathways mediated by TRAF-6. Immunohistochemical and Western blot analyses for TRAF-6, ASK-1, MEK-6, p38, Raf-1, MEK-1, ERK-1, ERK-2 and PSA were carried out in 5 samples of normal prostate gland, 24 samples of BPH and 19 samples of PC. Immunoreaction to TRAF-6 was found in the cytoplasm of epithelial cells of BPH and tumor cells of PC samples. For patients with the profile (TRAF-6+), optical densities revealed a weak immunoexpression of ASK-1 in PC compared to BPH patients. Whereas, immunoexpression to Raf-1 was higher in PC than in BPH. According to the expression of ASK-1 and Raf-1, two main profiles were identified: (TRAF-6+, ASK-1+, Raf-1+) and (TRAF-6+, ASK-1+, RAF-1-) in both BPH and PC. In addition, ASK-1/p38 axis expression was increased in BPH. Raf-1/ERK signaling pathway was increased in PC samples. On the other hand, representing of individual signaling protein expression enclosing each of p38 and ERK MAP Kinases according to TRAF-6+ showed a qualitative behavior of ASK61/p38 and Raf-1/ERK signaling pathways and a dynamic expression of PSA associated with immune and inflammatory process. These findings suggest that prostate epithelial cell could able an immune and inflammatory setting.
本研究旨在探讨 TRAF-6 介导的 ASK-1/p38 和 Raf-1/ERK MAPK 信号通路在前列腺上皮细胞免疫中的作用。采用免疫组化和 Western blot 法检测 TRAF-6、ASK-1、MEK-6、p38、Raf-1、MEK-1、ERK-1、ERK-2 和 PSA 在 5 例正常前列腺组织、24 例 BPH 和 19 例 PC 中的表达。TRAF-6 在 BPH 和 PC 组织的上皮细胞胞浆中呈阳性反应。PC 患者 TRAF-6 阳性组的 ASK-1 免疫反应强度较 BPH 患者减弱。PC 患者 Raf-1 的免疫反应强度高于 BPH 患者。根据 ASK-1 和 Raf-1 的表达情况，将 BPH 和 PC 患者分为 2 种主要表型：(TRAF-6+、ASK-1+、Raf-1+)和(TRAF-6+、ASK-1+、Raf-1-)。此外，BPH 患者的 ASK-1/p38 轴表达增加，PC 患者 Raf-1/ERK 信号通路表达增加。另一方面，根据 TRAF-6+，对包含 p38 和 ERK MAPK 在内的单个信号蛋白表达的分析显示，ASK61/p38 和 Raf-1/ERK 信号通路具有定性行为，PSA 与免疫和炎症过程相关，表达呈动态变化。这些发现提示前列腺上皮细胞可能具有免疫和炎症作用。
