直接在智利南部生态系统的环境样本中检测到膝沟藻的高遗传变异性。
High genetic variability of Alexandrium catenella directly detected in environmental samples from the Southern Austral Ecosystem of Chile.
机构信息
Marine Biotechnology Unit, Department of Oceanography, Faculty of Natural and Oceanographic Sciences, University of Concepción, Casilla 160-C, Concepción, Chile; Center for Oceanographic Research COPAS Sur-Austral, University of Concepción, Casilla 160-C, Concepción, Chile.
Marine Biotechnology Unit, Department of Oceanography, Faculty of Natural and Oceanographic Sciences, University of Concepción, Casilla 160-C, Concepción, Chile.
出版信息
Mar Pollut Bull. 2018 Feb;127:437-444. doi: 10.1016/j.marpolbul.2017.12.022. Epub 2017 Dec 21.
Chilean waters are often affected by Alexandrium catenella, one of the leading organisms behind Harmful Algae Blooms (HABs). Genetic variability for this species are commonly carried out from cultured samples, approach that may not accurately quantify genetic variability of this organism in the water column. In this study, genetic variability of A. catenella was determined by sequencing the rDNA region, in water samples from the Canal Puyuhuapi (South Austral Ecosystem of Chile). A. catenella was detected in 8,8% of samples analysed. All sequences obtained were A. catenella (Tamara complex group I), with three highly frequent haplotypes (34%), and twenty new haplotypes. These haplotypes increase the genetic variability from 2.8% to 3.14% in this area. Through this new method, genetic determination of A. catenella can accurately be monitored and ecological studies of this species can be implemented.
智利水域常受到塔玛亚历山大藻的影响，该藻是赤潮（HAB）的主要生物之一。该物种的遗传变异性通常是从培养样本中进行的，这种方法可能无法准确地量化水柱中该生物的遗传变异性。在这项研究中，通过对来自智利南澳大利亚生态系统的普尤瓦普伊海峡水样进行 rDNA 区域测序，确定了塔玛亚历山大藻的遗传变异性。在分析的 8.8%的样本中检测到了塔玛亚历山大藻。获得的所有序列均为塔玛亚历山大藻（塔玛复杂群 I），有三个高频单倍型（34%）和二十个新单倍型。这些单倍型使该地区的遗传变异性从 2.8%增加到 3.14%。通过这种新方法，可以准确地监测塔玛亚历山大藻的遗传情况，并对该物种进行生态研究。
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