硫酸角质素的高场核磁共振研究。鲨鱼软骨硫酸角质素的氢-1和碳-13化学位移归属
High-field n.m.r. studies of keratan sulphates. 1H and 13C assignments of keratan sulphate from shark cartilage.
作者信息
Cockin G H, Huckerby T N, Nieduszynski I A
出版信息
Biochem J. 1986 Jun 15;236(3):921-4. doi: 10.1042/bj2360921.
Abstract
Keratan sulphate was extracted from a shark/whale cartilage preparation and examined by 400 MHz 1H- and 100 MHz 13C-n.m.r. spectroscopy. Assignment of the majority of the resonances was facilitated by two-dimensional 13C-1H correlation by using a modified COLOC procedure and a COSY-45 experiment. The spectra are consistent with an N-acetyl-lactosamine repeating unit that is predominantly sulphated at C-6 of both galactose and N-acetylglucosamine. Gel chromatography of a keratanase digest of the shark keratan sulphate confirmed the high degree of galactose sulphation.
摘要
从鲨鱼/鲸鱼软骨制剂中提取硫酸角质素，并通过400 MHz 1H和100 MHz 13C核磁共振光谱进行检测。通过使用改良的COLOC程序和COSY-45实验进行二维13C-1H相关分析，有助于确定大多数共振峰。光谱结果与N-乙酰乳糖胺重复单元一致，该单元在半乳糖和N-乙酰葡糖胺的C-6位主要被硫酸化。鲨鱼硫酸角质素的角质酶消化产物的凝胶色谱分析证实了半乳糖硫酸化的高度。
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本文引用的文献
1
Polysulfated mucopolysaccharides of elasmobranch cartilage.板鳃亚纲软骨的多硫酸化粘多糖
J Biochem. 1961 Dec;50:546-7. doi: 10.1093/oxfordjournals.jbchem.a127489.
2
Ageing of human cartilage.人类软骨的老化
Nature. 1959 May 2;183(4670):1267-8. doi: 10.1038/1831267a0.
3
Mucopolysaccharides of costal cartilage.肋软骨的黏多糖
Science. 1958 Oct 17;128(3329):896. doi: 10.1126/science.128.3329.896.
4
The mucopolysaccharides of bovine cornea.牛角膜的黏多糖
J Biol Chem. 1953 Dec;205(2):611-6.
5
Age-related changes in the structure of the proteoglycan subunits from human articular cartilage.人类关节软骨蛋白聚糖亚基结构的年龄相关性变化。
J Biol Chem. 1980 Jan 10;255(1):217-24.
6
Isolation and characterization of dermatan sulphate proteoglycans from bovine sclera.从牛巩膜中分离并鉴定硫酸皮肤素蛋白聚糖
Biochem J. 1981 Jan 1;193(1):143-53. doi: 10.1042/bj1930143.
7
13C nuclear magnetic resonance suggests a flexible proteoglycan core protein.
J Biol Chem. 1981 Jul 25;256(14):7129-38.
8
Proteinase activity in chondroitin lyase (chondroitinase) and endo-beta-D-galactosidase (keratanase) preparations and a method to abolish their proteolytic effect on proteoglycan.软骨素裂解酶（软骨素酶）和内切-β-D-半乳糖苷酶（角质素酶）制剂中的蛋白酶活性以及消除它们对蛋白聚糖蛋白水解作用的方法。
Biochem J. 1980 Oct 1;191(1):203-7. doi: 10.1042/bj1910203.
9
Structures and immunochemical properties of oligosaccharides isolated from pig submaxillary mucins.从猪下颌粘蛋白中分离出的寡糖的结构和免疫化学性质。
J Biol Chem. 1968 Feb 10;243(3):616-26.
10
Studies on keratosulfates. Methylation, desulfation, and acid hydrolysis studies on old human rib cartilage keratosulfate.硫酸角质素的研究。对陈旧人肋软骨硫酸角质素的甲基化、脱硫及酸水解研究。
J Biol Chem. 1968 Mar 10;243(5):1052-9.
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