Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Department of Microbial Natural Products, Helmholtz Centre for Infection Research and Department of Pharmaceutical Biotechnology, Saarland University, Campus E8.1, 66123, Saarbrücken, Germany.
German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, 38124, Braunschweig, Germany.
Nat Commun. 2018 Feb 23;9(1):803. doi: 10.1038/s41467-018-03184-1.
Some bacterial clades are important sources of novel bioactive natural products. Estimating the magnitude of chemical diversity available from such a resource is complicated by issues including cultivability, isolation bias and limited analytical data sets. Here we perform a systematic metabolite survey of ~2300 bacterial strains of the order Myxococcales, a well-established source of natural products, using mass spectrometry. Our analysis encompasses both known and previously unidentified metabolites detected under laboratory cultivation conditions, thereby enabling large-scale comparison of production profiles in relation to myxobacterial taxonomy. We find a correlation between taxonomic distance and the production of distinct secondary metabolite families, further supporting the idea that the chances of discovering novel metabolites are greater by examining strains from new genera rather than additional representatives within the same genus. In addition, we report the discovery and structure elucidation of rowithocin, a myxobacterial secondary metabolite featuring an uncommon phosphorylated polyketide scaffold.
一些细菌类群是新型生物活性天然产物的重要来源。估计此类资源中可用的化学多样性的大小受到可培养性、分离偏差和有限的分析数据集等问题的影响。在这里，我们使用质谱法对约 2300 株粘球菌目细菌菌株进行了系统的代谢物调查，该目是天然产物的重要来源。我们的分析包括在实验室培养条件下检测到的已知和以前未识别的代谢物，从而能够大规模比较与粘细菌分类学有关的生产概况。我们发现分类距离与不同次生代谢物家族的产生之间存在相关性，这进一步支持了这样一种观点，即通过检查来自新属的菌株而不是同一属的更多代表菌株，发现新的代谢物的机会更大。此外，我们还报告了罗维西菌素的发现和结构阐明，这是一种粘细菌次生代谢物，具有罕见的磷酸化聚酮骨架。
