Katakai Tomoya
Department of Immunology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
Methods Mol Biol. 2018;1763:29-42. doi: 10.1007/978-1-4939-7762-8_4.
Live imaging using various microscopic technologies is an indispensable tool for investigating the dynamic nature of immune cells. One of the most powerful techniques is the two-photon laser-scanning microscopy (TP-LSM), which has various advantages in observing deep tissues in vivo. Interstitial T cell migration in the lymph node (LN) is a phenomenon intensively examined using TP-LSM in the field of immunology. Intravital and explant methods have been standards for imaging T cell behaviors in the LN, though there are several limitations. Live imaging of LN slices, an LN explant sliced by a vibratome to expose tissue parenchyma, could provide an alternative approach with technical advantages for an in-depth analysis of interstitial T cell migration in vivo.
使用各种显微技术进行实时成像，是研究免疫细胞动态特性不可或缺的工具。最强大的技术之一是双光子激光扫描显微镜（TP-LSM），它在体内观察深部组织方面具有多种优势。淋巴结（LN）中的间质T细胞迁移是免疫领域中使用TP-LSM深入研究的现象。尽管存在一些局限性，但活体和外植体方法一直是LN中T细胞行为成像的标准方法。LN切片的实时成像，即通过振动切片机切割的LN外植体以暴露组织实质，可以为体内间质T细胞迁移的深入分析提供一种具有技术优势的替代方法。
