Egawa Gyohei, Honda Tetsuya, Kabashima Kenji
Department of Dermatology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Methods Mol Biol. 2018;1763:75-85. doi: 10.1007/978-1-4939-7762-8_8.
A variety of immune cells are involved in cutaneous immune responses. Over the last decade, intravital imaging has become an important technique used to capture the dynamic behavior of immune cells in the physiological context. In this chapter, we describe essential techniques for visualizing immune cells in the skin, focusing on the contact hypersensitivity response. Using fluorescent dyes and transgenic reporter animals, many kinds of immune cells and skin components can be visualized in three dimensions and in a noninvasive manner.
多种免疫细胞参与皮肤免疫反应。在过去十年中，活体成像已成为一种重要技术，用于捕捉生理环境中免疫细胞的动态行为。在本章中，我们描述了可视化皮肤中免疫细胞的基本技术，重点是接触性超敏反应。使用荧光染料和转基因报告动物，可以以三维和非侵入性方式可视化多种免疫细胞和皮肤成分。
