Singapore Immunology Network (SIgN) and Institute of Medical Biology (IMB), Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore.
Department of Dermatology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Cell Immunol. 2020 Apr;350:103813. doi: 10.1016/j.cellimm.2018.05.006. Epub 2018 May 26.
Various immune cells are present in the skin and modulate the cutaneous immune response. In order to capture such dynamic phenomena, intravital imaging is an important technique and there is a possibility to provide substantial information that is not available using conventional histological analysis. Multiphoton microscope enable direct, three-dimensional, minimally invasive imaging of biological samples with high spatiotemporal resolution, and now become the main method for intravital imaging studies. Here, we will introduce the latest knowledge obtained by intravital imaging of the skin.
各种免疫细胞存在于皮肤中，并调节皮肤免疫反应。为了捕捉这种动态现象，活体成像技术是一种重要的技术，有可能提供传统组织学分析无法获得的大量信息。多光子显微镜能够以高时空分辨率直接、三维、微创成像生物样本，现在成为活体成像研究的主要方法。在这里，我们将介绍通过皮肤活体成像获得的最新知识。
