Dinsmore C E, Goss R J, Lenz M E, Thonar E J
Calcif Tissue Int. 1986 Oct;39(4):244-7. doi: 10.1007/BF02555212.
A sensitive and highly specific enzyme-linked immunosorbent assay with an inhibition step was used to monitor the concentration of keratan sulfate, a cartilage-related glycosaminoglycan, in the serum of three adult male deer. During the course of one complete annual antler regeneration cycle, keratan sulfate levels were found to fluctuate predictably in relation to the growth and maturation of the antlers: levels are substantially elevated during the growth phase and drop precipitously when growth ceases and the antlers become fully mineralized. In addition, an unanticipated elevation of serum keratan sulfate was observed in early spring prior to casting of the preceding year's antlers and the initiation of regrowth. This suggests that changes in cartilage metabolism occur concomitantly, with this phase of the antlerogenic cycle. These results show predictable and physiologically regulated variation in serum keratan sulfate levels which correlate directly with specific phases of the antler regeneration cycle. Furthermore, the findings provide additional support for the assertion that measurements of keratan sulfate levels in serum can provide useful information about changes in cartilage metabolism in normal as well as diseased states.
采用一种带有抑制步骤的灵敏且高度特异的酶联免疫吸附测定法，来监测三只成年雄性鹿血清中硫酸角质素（一种与软骨相关的糖胺聚糖）的浓度。在一个完整的年度鹿角再生周期中，发现硫酸角质素水平会随着鹿角的生长和成熟而呈现可预测的波动：在生长阶段大幅升高，当生长停止且鹿角完全矿化时急剧下降。此外，在脱落前一年的鹿角并开始重新生长之前的早春，观察到血清硫酸角质素意外升高。这表明软骨代谢的变化与鹿角发生周期的这一阶段同时出现。这些结果显示血清硫酸角质素水平存在可预测且受生理调节的变化，这些变化与鹿角再生周期的特定阶段直接相关。此外，这些发现为以下论断提供了更多支持，即血清中硫酸角质素水平的测量可为正常及患病状态下软骨代谢的变化提供有用信息。
