Young M R, Newby M, Wepsic H T
Cancer Res. 1987 Jan 1;47(1):100-5.
Mice bearing a metastatic variant of Lewis lung carcinoma (LLC-C3) were studied to determine if there might be a relationship between tumor induced hematopoiesis and immune suppression. Growth of LLC-C3 in C57BL/6 mice corresponded with increased hematopoiesis and an increase in the proportion of monocytes in the peripheral blood, spleen, and bone marrow. The LLC-C3 cells secreted colony stimulating factor activity and, thus, could have directly stimulated the hematopoiesis in the hosts. As tumor growth progressed, the bone marrow of the tumor bearing mice became suppressive to T-cell blastogenesis. The bone marrow suppressor cells obtained from mice bearing large (greater than 3 g) LLC-C3 tumors were nonadherent to nylon wool, sensitive to treatment with L-leucine methyl ester, insensitive to treatment with anti-Thy-1.2 and complement, and mediated their suppression through an indomethacin sensitive mechanism. Secretion of colony stimulating factor activity by the LLC-C3 cells could have induced the appearance of the bone marrow suppressor cells since normal bone marrow cells which were cultured in the presence of LLC-C3 culture supernatants had an increased proportion of monocytes and were suppressive to T-lymphocyte blastogenesis. Our results suggest that the colony stimulating factor activity produced by LLC-C3 cells stimulates hematopoiesis which, in turn, could result in the appearance of bone marrow suppressor cells.
对携带Lewis肺癌转移变体（LLC-C3）的小鼠进行了研究，以确定肿瘤诱导的造血与免疫抑制之间是否可能存在关联。LLC-C3在C57BL/6小鼠体内的生长与造血增加以及外周血、脾脏和骨髓中单核细胞比例的增加相对应。LLC-C3细胞分泌集落刺激因子活性，因此可能直接刺激宿主的造血。随着肿瘤生长的进展，荷瘤小鼠的骨髓对T细胞增殖产生抑制作用。从携带大（大于3g）LLC-C3肿瘤的小鼠获得的骨髓抑制细胞不黏附于尼龙毛，对L-亮氨酸甲酯处理敏感，对抗Thy-1.2和补体处理不敏感，并通过一种对吲哚美辛敏感的机制介导其抑制作用。LLC-C3细胞分泌集落刺激因子活性可能诱导了骨髓抑制细胞的出现，因为在LLC-C3培养上清液存在的情况下培养的正常骨髓细胞中单核细胞比例增加，并且对T淋巴细胞增殖具有抑制作用。我们的结果表明，LLC-C3细胞产生的集落刺激因子活性刺激造血，进而可能导致骨髓抑制细胞的出现。
