利用Cas9n引导的tRNA腺苷脱氨酶在水稻中实现高效的A·T到G·C碱基编辑 - Suppr

Highly Efficient A·T to G·C Base Editing by Cas9n-Guided tRNA Adenosine Deaminase in Rice.

作者信息

Yan Fang, Kuang Yongjie, Ren Bin, Wang Jingwen, Zhang Dawei, Lin Honghui, Yang Bing, Zhou Xueping, Zhou Huanbin

机构信息

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Ministry of Education Key Laboratory of Bio-Resource and Eco-Environment, College of Life Sciences, Sichuan University, Chengdu 610065, China.

出版信息

Mol Plant. 2018 Apr 2;11(4):631-634. doi: 10.1016/j.molp.2018.02.008. Epub 2018 Feb 22.

DOI:10.1016/j.molp.2018.02.008

PMID:29476918

Abstract

摘要

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Highly Efficient A·T to G·C Base Editing by Cas9n-Guided tRNA Adenosine Deaminase in Rice.

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Highly Efficient A·T to G·C Base Editing by Cas9n-Guided tRNA Adenosine Deaminase in Rice.

作者信息

Yan Fang, Kuang Yongjie, Ren Bin, Wang Jingwen, Zhang Dawei, Lin Honghui, Yang Bing, Zhou Xueping, Zhou Huanbin

机构信息

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

出版信息

Mol Plant. 2018 Apr 2;11(4):631-634. doi: 10.1016/j.molp.2018.02.008. Epub 2018 Feb 22.

DOI:10.1016/j.molp.2018.02.008

PMID:29476918

Abstract

摘要

相似文献

Highly Efficient A·T to G·C Base Editing by Cas9n-Guided tRNA Adenosine Deaminase in Rice.

Mol Plant. 2018 Apr 2;11(4):631-634. doi: 10.1016/j.molp.2018.02.008. Epub 2018 Feb 22.

Multiplex nucleotide editing by high-fidelity Cas9 variants with improved efficiency in rice.

BMC Plant Biol. 2019 Nov 21;19(1):511. doi: 10.1186/s12870-019-2131-1.

Structure-guided engineering of adenine base editor with minimized RNA off-targeting activity.

Nat Commun. 2021 Apr 16;12(1):2287. doi: 10.1038/s41467-021-22519-z.

Off-Target Editing by CRISPR-Guided DNA Base Editors.

Biochemistry. 2019 Sep 10;58(36):3727-3734. doi: 10.1021/acs.biochem.9b00573. Epub 2019 Aug 26.

Highly efficient RNA-guided base editing in rabbit.

Nat Commun. 2018 Jul 13;9(1):2717. doi: 10.1038/s41467-018-05232-2.

A CRISPR/Cas9 toolkit for efficient targeted base editing to induce genetic variations in rice.

Sci China Life Sci. 2017 May;60(5):516-519. doi: 10.1007/s11427-016-0406-x. Epub 2017 Mar 3.

Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion.

Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.

A Multiplexed CRISPR/Cas9 Editing System Based on the Endogenous tRNA Processing.

Methods Mol Biol. 2019;1917:63-73. doi: 10.1007/978-1-4939-8991-1_5.

Evaluating and Enhancing Target Specificity of Gene-Editing Nucleases and Deaminases.

Annu Rev Biochem. 2019 Jun 20;88:191-220. doi: 10.1146/annurev-biochem-013118-111730. Epub 2019 Mar 18.

Engineering Introns to Express RNA Guides for Cas9- and Cpf1-Mediated Multiplex Genome Editing.

Mol Plant. 2018 Apr 2;11(4):542-552. doi: 10.1016/j.molp.2018.02.005. Epub 2018 Feb 17.

引用本文的文献

Emerging trends in transgene-free crop development: insights into genome editing and its regulatory overview.

Plant Mol Biol. 2025 Jul 9;115(4):84. doi: 10.1007/s11103-025-01600-x.

CRISPR-Cas9 mediated enhancement of abiotic stress resilience in tomato: a comprehensive review of target genes.

Mol Biol Rep. 2025 Jun 3;52(1):538. doi: 10.1007/s11033-025-10634-9.

A comprehensive all-in-one CRISPR toolbox for large-scale screens in plants.

Plant Cell. 2025 Apr 2;37(4). doi: 10.1093/plcell/koaf081.

Emerging applications of gene editing technologies for the development of climate-resilient crops.

Front Genome Ed. 2025 Mar 10;7:1524767. doi: 10.3389/fgeed.2025.1524767. eCollection 2025.

All Roads Lead to Rome: Pathways to Engineering Disease Resistance in Plants.

Adv Sci (Weinh). 2025 Feb;12(5):e2412223. doi: 10.1002/advs.202412223. Epub 2024 Dec 18.

CRISPR/Cas system-mediated base editing in crops: recent developments and future prospects.

Plant Cell Rep. 2024 Oct 25;43(11):271. doi: 10.1007/s00299-024-03346-0.

Biotechnological studies towards improvement of finger millet using multi-omics approaches.

Funct Integr Genomics. 2024 Sep 2;24(5):148. doi: 10.1007/s10142-024-01438-4.

High performance TadA-8e derived cytosine and dual base editors with undetectable off-target effects in plants.

Nat Commun. 2024 Jun 14;15(1):5103. doi: 10.1038/s41467-024-49473-w.

Diverse nucleotide substitutions in rice base editing mediated by novel TadA variants.

Plant Commun. 2024 Aug 12;5(8):100926. doi: 10.1016/j.xplc.2024.100926. Epub 2024 May 8.

Engineering disease-resistant plants with alternative translation efficiency by switching uORF types through CRISPR.

Sci China Life Sci. 2024 Aug;67(8):1715-1726. doi: 10.1007/s11427-024-2588-9. Epub 2024 Apr 26.

Highly Efficient A·T to G·C Base Editing by Cas9n-Guided tRNA Adenosine Deaminase in Rice.

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Highly Efficient A·T to G·C Base Editing by Cas9n-Guided tRNA Adenosine Deaminase in Rice.

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