Wu Chia-Lin, Chang Ching-Ching, Wu Jie-Kai, Chiang Meng-Hsuan, Yang Chu-Huai, Chiang Hsueh-Cheng
Department of Biochemistry and Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Department of Neurology, Chang Gung Memorial Hospital, Linkou, Taiwan.
Department of Biochemistry and Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Neurobiol Learn Mem. 2018 Apr;150:13-19. doi: 10.1016/j.nlm.2018.02.015. Epub 2018 Feb 22.
Glucose catabolism, also known as glycolysis, is important for energy generation and involves a sequence of enzymatic reactions that convert a glucose molecule into two pyruvate molecules. The glycolysis process generates adenosine triphosphate as a byproduct. In this study, we investigated whether glycolysis plays a role in maintaining neuronal functions in the Drosophila mushroom bodies (MBs), which are generally accepted to be an olfactory learning and memory center. Our data showed that individual knockdown of glycolytic enzymes in the MBs, including hexokinase (HexA), phosphofructokinase (Pfk), or pyruvate kinase (PyK), disrupts olfactory memory. Whole-mount brain immunostaining indicated that pyruvate kinase is strongly expressed in the MB αβ, α'β', and γ neuron subsets. We conclude that HexA, Pfk, and PyK are required in each MB neuron subset for olfactory memory formation. Our data therefore indicates that glucose catabolism in the MBs is important for olfactory memory formation in Drosophila.
葡萄糖分解代谢，也称为糖酵解，对能量产生很重要，它涉及一系列将葡萄糖分子转化为两个丙酮酸分子的酶促反应。糖酵解过程会产生三磷酸腺苷作为副产品。在本研究中，我们调查了糖酵解是否在果蝇蘑菇体（MBs）中维持神经元功能方面发挥作用，果蝇蘑菇体通常被认为是嗅觉学习和记忆中心。我们的数据表明，在蘑菇体中单独敲低糖酵解酶，包括己糖激酶（HexA）、磷酸果糖激酶（Pfk）或丙酮酸激酶（PyK），会破坏嗅觉记忆。全脑免疫染色表明丙酮酸激酶在蘑菇体αβ、α'β'和γ神经元亚群中强烈表达。我们得出结论，每个蘑菇体神经元亚群中的HexA、Pfk和PyK是嗅觉记忆形成所必需 的。因此，我们的数据表明，蘑菇体中的葡萄糖分解代谢对果蝇的嗅觉记忆形成很重要。
