Gatenby P A, Engleman E G
Clin Exp Immunol. 1986 Aug;65(2):401-8.
A suppressor-amplifier circuit controlling the generation of plaque-forming cells has been demonstrated previously by us in the autologous mixed leucocyte reaction. Thus, after Leu-3+ dependent activation in an initial culture, radio-resistant Leu-2+, DR+ suppressor-amplifier cells can potently suppress Leu-3+ dependent generation of antibody forming cells, but only in the presence of fresh Leu-2+, DR- suppressor-effector cells. The target of this suppressor-amplifier circuit has been examined in a triple culture system. Suppressor-amplifier cells were activated in an initial culture; Leu-2+ cells were separated from this initial culture and transferred to an intermediate culture. After 72 h Leu-3+ helper T cells were separated from this culture and tested for their ability to support the generation of antibody-forming cells in a third culture. Such helper T cells were unable to support antibody synthesis when cultured with fresh B cells. In contrast, B cells from the intermediate culture could be activated to produce antibodies, when cultured with a fresh source of T cell help. These findings are consistent with the helper T cells being the target of the suppressor-amplifier circuit.
我们之前已经在自体混合淋巴细胞反应中证实了一种控制噬斑形成细胞产生的抑制-放大电路。因此，在初始培养中经Leu-3+依赖性激活后，抗辐射的Leu-2+、DR+抑制-放大细胞能够有效抑制Leu-3+依赖性抗体形成细胞的产生，但前提是存在新鲜的Leu-2+、DR-抑制效应细胞。在三重培养系统中对这种抑制-放大电路的靶标进行了检测。抑制-放大细胞在初始培养中被激活；将Leu-2+细胞从该初始培养中分离出来并转移至中间培养。72小时后，将Leu-3+辅助性T细胞从该培养中分离出来，并检测它们在第三种培养中支持抗体形成细胞产生的能力。当与新鲜B细胞一起培养时，这种辅助性T细胞无法支持抗体合成。相反，当与新鲜的T细胞辅助来源一起培养时，来自中间培养的B细胞可被激活以产生抗体。这些发现与辅助性T细胞是抑制-放大电路的靶标这一观点一致。
