Engleman E G, Benike C J, Grumet F C, Evans R L
J Immunol. 1981 Nov;127(5):2124-9.
This study was directed at determining the major histocompatibility complex (MHC) antigens recognized by helper (Leu-3) and suppressor-cytotoxic (Leu-2) T lymphocyte subsets in man. These 2 subsets were isolated from peripheral blood with monoclonal antibodies and challenged in vitro with various stimuli. Only Leu-3 cells proliferated in response to autologous nonrosetting cells and soluble antigens, suggesting that helper but not suppressor-cytotoxic T cells recognize autologous HLA-DR antigen. Furthermore, only Leu-3 T cells responded to allogeneic DR antigen; this was shown in reactions between 2 siblings who were HLA-identical except for a single disparity at HLA-DR caused by a crossover event. Leu-3 cells activated in primary allogeneic mixed leukocyte reactions (MLR) responded equally in secondary allogeneic MLR to the priming cells and to cells that were identical at HLA-DR but discordant at HLA-A, B, and C to the priming cell. The antigens responsible for stimulating Leu-2 cells in allogeneic MLR were not identified, although the results are compatible with a role for HLA-A and B antigens and exclude a dominant role for HLA-DR. These data indicate that the helper and suppressor-cytotoxic T cell subsets in man respond differentially to MHC antigens in a manner analogous to the murine Lyt-2- 3- and Lyt-2+ 3+ populations.
本研究旨在确定人类辅助性（Leu-3）和抑制性细胞毒性（Leu-2）T淋巴细胞亚群所识别的主要组织相容性复合体（MHC）抗原。这两个亚群通过单克隆抗体从外周血中分离出来，并在体外接受各种刺激进行挑战。只有Leu-3细胞对自体非凝集细胞和可溶性抗原产生增殖反应，这表明辅助性T细胞而非抑制性细胞毒性T细胞识别自体HLA-DR抗原。此外，只有Leu-3 T细胞对同种异体DR抗原产生反应；这在两名HLA相同但因交叉事件导致HLA-DR存在单个差异的同胞之间的反应中得到了证实。在初次同种异体混合淋巴细胞反应（MLR）中被激活的Leu-3细胞，在二次同种异体MLR中对启动细胞以及在HLA-DR相同但在HLA-A、B和C与启动细胞不一致的细胞产生同等反应。尽管结果与HLA-A和B抗原的作用相符且排除了HLA-DR的主导作用，但在同种异体MLR中刺激Leu-2细胞的抗原尚未确定。这些数据表明，人类辅助性和抑制性细胞毒性T细胞亚群对MHC抗原的反应存在差异，其方式类似于小鼠的Lyt-2- 3-和Lyt-2+ 3+群体。
