Kralisz U, Kotelba-Witkowska B
Cytobios. 1986;48(192):25-30.
The activity of two membrane-bound enzymes of human platelets subjected to DMSO and a freezing-thawing process were analysed. Following treatment of fresh platelets with DMSO (5-20%) quantitative differences of AChE and 'ecto-ATPase' activities were seen. After cryopreservation of platelets in 5% DMSO the enzymatic activities of AChE and Mg2+-ATPase were no different from those obtained for fresh platelets. The lack of any changes in the activities of the enzymes of frozen platelets subjected to a washing procedure to remove DMSO, indicates that the mechanism of the DMSO-induced effect is reversible and that freezing-thawing process had no additional detrimental effects.
分析了经二甲基亚砜（DMSO）处理及冻融过程的人血小板中两种膜结合酶的活性。用DMSO（5%-20%）处理新鲜血小板后，乙酰胆碱酯酶（AChE）和“外切ATP酶”活性出现了定量差异。血小板在5% DMSO中冻存后，AChE和Mg2+-ATP酶的酶活性与新鲜血小板无异。对经洗涤程序以去除DMSO的冻存血小板的酶活性未发现任何变化，这表明DMSO诱导效应的机制是可逆的，且冻融过程没有额外的有害影响。
