Department of Biotechnologies and Biosciences, University of Milano-Bicocca, P.zza della Scienza 2, 20126 Milan, Italy.
Department of Molecular Biochemistry and Pharmacology, IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri", Via G. La Masa 19, 20156 Milano, Italy.
Food Chem. 2018 Jun 30;252:171-180. doi: 10.1016/j.foodchem.2018.01.075. Epub 2018 Jan 12.
To identify food and beverages that provide the regular intake of natural compounds capable of interfering with toxic amyloidogenic aggregates, we developed an experimental protocol that combines NMR spectroscopy and atomic force microscopy, in vitro biochemical and cell assays to detect anti-Aβ molecules in natural edible matrices. We applied this approach to investigate the potential anti-amyloidogenic properties of coffee and its molecular constituents. Our data showed that green and roasted coffee extracts and their main components, 5-O-caffeoylquinic acid and melanoidins, can hinder Aβ on-pathway aggregation and toxicity in a human neuroblastoma SH-SY5Y cell line. Coffee extracts and melanoidins also counteract hydrogen peroxide- and rotenone-induced cytotoxicity and modulate some autophagic pathways in the same cell line.
为了鉴定能够经常摄入、具有干扰毒性淀粉样蛋白聚集能力的天然化合物的食物和饮料，我们开发了一种实验方案，将核磁共振波谱和原子力显微镜、体外生化和细胞测定相结合，以检测天然可食用基质中的抗 Aβ 分子。我们应用这种方法来研究咖啡及其分子成分的潜在抗淀粉样蛋白特性。我们的数据表明，绿原酸和咖啡酸及其主要成分 5-O-咖啡酰奎宁酸和类黑素可以阻止人神经母细胞瘤 SH-SY5Y 细胞系中 Aβ 的通路聚集和毒性。咖啡提取物和类黑素还可以抵抗过氧化氢和鱼藤酮诱导的细胞毒性，并调节同一细胞系中的一些自噬途径。
