School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
J Environ Sci (China). 2018 Feb;64:207-215. doi: 10.1016/j.jes.2017.06.001. Epub 2017 Jun 15.
In this paper, the inhibition of methanogens by phenol in coal gasification wastewater (CGW) was investigated by both anaerobic toxicity tests and a lab-scale anaerobic biofilter reactor (AF). The anaerobic toxicity tests indicated that keeping the phenol concentration in the influent under 280mg/L could maintain the methanogenic activity. In the AF treating CGW, the result showed that adding glucose solution as co-substrate could be beneficial for the quick start-up of the reactor. The effluent chemical oxygen demand (COD) and total phenol reached 1200 and 100mg/L, respectively, and the methane production rate was 175mLCH/gCOD/day. However, if the concentration of phenol was increased, the inhibition of anaerobic micro-organisms was irreversible. The threshold of total phenol for AF operation was 200-250mg/L. The extracellular polymeric substances (EPS) and particle size distribution of anaerobic granular sludge in the different stages were also examined, and the results indicated that the influence of toxicity in the system was more serious than its effect on flocculation of EPS. Moreover, the proportion of small size anaerobic granular sludge gradually increased from 10.2% to 34.6%. The results of high through-put sequencing indicated that the abundance of the Chloroflexi and Planctomycetes was inhibited by the toxicity of the CGW, and some shifts in the microbial community were observed at different stages.
本文通过厌氧毒性试验和实验室规模的厌氧生物滤池反应器（AF）研究了苯酚对煤气化废水中产甲烷菌的抑制作用。厌氧毒性试验表明，将进水苯酚浓度保持在 280mg/L 以下，可以维持产甲烷菌的活性。在 AF 处理 CGW 的过程中，结果表明添加葡萄糖溶液作为共基质有利于反应器的快速启动。出水化学需氧量（COD）和总酚分别达到 1200 和 100mg/L，甲烷产率为 175mLCH/gCOD/天。然而，如果苯酚浓度增加，对厌氧微生物的抑制作用是不可逆的。AF 运行的总酚阈值为 200-250mg/L。还检查了不同阶段厌氧颗粒污泥的胞外聚合物（EPS）和颗粒大小分布，结果表明系统中毒性的影响比 EPS 絮凝的影响更严重。此外，小尺寸厌氧颗粒污泥的比例从 10.2%逐渐增加到 34.6%。高通量测序结果表明，CGW 的毒性抑制了绿弯菌门和浮霉菌门的丰度，并且在不同阶段观察到微生物群落的一些变化。
