Xie Fang, Fei Xiang, Zhang Ming-Bo, Zhang Yan, Wang Hong-Wei, Tang Jie, Tang Wen-Bo, Luo Yu-Kun
Department of Ultrasound, Chinese PLA General Hospital, Beijing, China.
Department of Ultrasound, Chinese PLA General Hospital, Beijing, China.
Ultrasound Med Biol. 2018 May;44(5):1053-1062. doi: 10.1016/j.ultrasmedbio.2018.01.004. Epub 2018 Feb 22.
The aim of this study was to evaluate microvascular perfusion after liver ischemia-reperfusion injury (IRI) in rabbits using the "flash-replenishment" method of contrast-enhanced ultrasound (CEUS) perfusion imaging. Twenty-eight rabbits underwent either 30, 60 or 90 min of ischemia and 120 min of reperfusion. CEUS perfusion imaging was performed using the "flash-replenishment" model, and hepatic microvascular perfusion parameters, including peak intensity (PI), area under the curve (AUC), and hepatic artery-to-vein transit time (HA-HVTT), were calculated. Prolonged ischemia upregulated intracellular adhesion molecule-1 (ICAM-1), alanine transaminase (ALT) and aspartate transaminase (AST) levels. Longer ischemia decreased PI and AUC, but increased HA-HVTT. The perfusion parameters were significantly correlated with Suzuki's pathology scores and ALT and AST levels. The "flash-replenishment" method of CEUS perfusion imaging is an accurate and non-invasive method for evaluating hepatic microvascular perfusion and provides a valuable experimental basis for early prediction of liver IRI damage after liver transplantation or liver resection.
本研究的目的是采用对比增强超声（CEUS）灌注成像的“快速补充”方法，评估兔肝脏缺血再灌注损伤（IRI）后的微血管灌注情况。28只兔分别经历了30、60或90分钟的缺血及120分钟的再灌注。使用“快速补充”模型进行CEUS灌注成像，并计算肝脏微血管灌注参数，包括峰值强度（PI）、曲线下面积（AUC）以及肝动脉至静脉渡越时间（HA-HVTT）。长时间缺血会上调细胞间黏附分子-1（ICAM-1）、丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）水平。缺血时间延长会降低PI和AUC，但会增加HA-HVTT。灌注参数与铃木病理学评分以及ALT和AST水平显著相关。CEUS灌注成像的“快速补充”方法是评估肝脏微血管灌注的一种准确且无创的方法，为肝移植或肝切除术后早期预测肝脏IRI损伤提供了有价值的实验依据。
