Hickstein D D, Smith A, Fisher W, Beatty P G, Schwartz B R, Harlan J M, Root R K, Locksley R M
J Immunol. 1987 Jan 15;138(2):513-9.
We used the HL-60 human promyelocytic leukemia cell line to analyze the surface expression of a family of adherence-related leukocyte surface antigens during myeloid differentiation. These antigens are composed of discrete alpha subunits, designated alpha L, alpha M, and alpha X, that are each noncovalently associated with a common beta subunit. Monoclonal antibodies directed against the individual subunits served as markers in both indirect immunofluorescence studies and immunoprecipitations from HL-60 cells differentiated preferentially towards mature granulocytes (DMSO, retinoic acid) or monocyte/macrophages (PMA, vitamin D3). In undifferentiated HL-60 cells, the alpha L and alpha X subunits were constitutively expressed, whereas the alpha M subunit was not. Differentiation of HL-60 cells along the granulocytic pathway with DMSO resulted in a marked increase in alpha M and minimal increases in alpha L and alpha X. The phenotypic expression of these antigens on DMSO-treated HL-60 cells closely resembled that on normal circulating PMN. Differentiation along the monocyte/macrophage pathway when using PMA or vitamin D3 resulted in major increases in alpha L and alpha X expression, as well as alpha M. These changes resulted in a surface phenotype characteristic of that present on human monocyte-derived macrophages. Triggering of undifferentiated HL-60 cells with PMA caused no increase in subunit expression, whereas stimulation of DMSO-differentiated HL-60 cells with PMA produced more than a 1.5-fold enhancement of both the alpha M and alpha X subunits, and stimulation of human PMN with PMA increased the surface expression of alpha M more than fourfold and alpha X subunit twofold. Stimulation with PMA produced no change in expression of the alpha L subunit in any of the three cell populations. These results indicate that the alpha subunits of this glycoprotein family can be selectively regulated during in vitro differentiation of a human promyelocytic leukemia cell line. Second, DMSO-differentiated HL-60 cells and human PMN possessed an intracellular pool of alpha M and alpha X, but not alpha L, that could be translocated to the surface. Thus, despite structural and functional relationships among the alpha subunits in this glycoprotein family, they undergo disparate surface expression and intracellular regulation during differentiation.
我们使用HL-60人早幼粒细胞白血病细胞系来分析髓系分化过程中一组与黏附相关的白细胞表面抗原的表面表达情况。这些抗原由离散的α亚基组成，分别命名为αL、αM和αX，每个亚基都与一个共同的β亚基非共价结合。针对各个亚基的单克隆抗体在间接免疫荧光研究以及从优先分化为成熟粒细胞（二甲基亚砜、视黄酸）或单核细胞/巨噬细胞（佛波酯、维生素D3）的HL-60细胞中进行免疫沉淀时用作标记物。在未分化的HL-60细胞中，αL和αX亚基组成性表达，而αM亚基不表达。用二甲基亚砜使HL-60细胞沿粒细胞途径分化导致αM显著增加，αL和αX略有增加。这些抗原在经二甲基亚砜处理的HL-60细胞上的表型表达与正常循环中的中性粒细胞非常相似。使用佛波酯或维生素D3沿单核细胞/巨噬细胞途径分化导致αL和αX表达以及αM大幅增加。这些变化导致了人单核细胞衍生巨噬细胞所具有的表面表型特征。用佛波酯刺激未分化的HL-60细胞不会导致亚基表达增加，而用佛波酯刺激经二甲基亚砜分化的HL-60细胞会使αM和αX亚基增加超过1.5倍，用佛波酯刺激人中性粒细胞会使αM的表面表达增加超过四倍，αX亚基增加两倍。用佛波酯刺激在这三种细胞群体中的任何一种中都不会导致αL亚基表达发生变化。这些结果表明，在人早幼粒细胞白血病细胞系的体外分化过程中，这个糖蛋白家族的α亚基可以被选择性调节。其次，经二甲基亚砜分化的HL-60细胞和人中性粒细胞具有αM和αX的细胞内池，但不具有αL，它们可以转运到表面。因此，尽管这个糖蛋白家族中α亚基之间存在结构和功能关系，但它们在分化过程中经历不同的表面表达和细胞内调节。
