Unic Adriana, Nikolac Gabaj Nora, Miler Marijana, Culej Jelena, Lisac Adrijana, Horvat Anita, Vrkic Nada
University Department of Chemistry, Medical School University Hospital Sestre Milosrdnice, Zagreb, Croatia.
Medical Biochemistry Laboratory, Specialised Hospital for the Extended Medical Treatment, Duga Resa, Croatia.
J Clin Lab Anal. 2018 Jun;32(5):e22390. doi: 10.1002/jcla.22390. Epub 2018 Feb 25.
Study was performed in order: (i) to assess the comparability of glucose, bilirubin, hemoglobin, leukocyte esterase, and protein; (ii) to assess accuracy of glucose, bilirubin, hemoglobin, leukocyte esterase, and protein; and (iii) to evaluate interference of ascorbic acid on the glucose, bilirubin, hemoglobin, and nitrite determination using 2 different dipsticks: iChem Velocity, Iris Diagnostics and Combur-10M, Roche Diagnostics.
Random urine specimens were included in the study. Comparability, accuracy, and ascorbic acid interference testing were performed.
Obtained results have shown almost perfect agreement for all parameters between 2 dipsticks in samples with negative ascorbic acid. Agreement in samples with positive ascorbic acid was not acceptable for bilirubin, protein, nitrite, and hemoglobin. Accuracy was not acceptable for hemoglobin and leukocyte esterase on both dipsticks. Ascorbic acid interference examination has shown that intensity of interference differs between dipsticks. Ascorbic acid interferes with glucose, hemoglobin, nitrite, and bilirubin at different concentrations causing false-negative results.
Obtained results indicate that it is necessary to determine diagnostic accuracy of used dipstick in order to define purpose of urinalysis. It is very important to choose dipstick with ascorbic acid indicator and to examine ascorbic acid impact on dipstick analytes independently of manufacturer claims.
开展本研究的目的如下：（i）评估葡萄糖、胆红素、血红蛋白、白细胞酯酶和蛋白质的可比性；（ii）评估葡萄糖、胆红素、血红蛋白、白细胞酯酶和蛋白质的准确性；（iii）使用两种不同的试纸条（iChem Velocity，Iris Diagnostics和Combur - 10M，Roche Diagnostics）评估抗坏血酸对葡萄糖、胆红素、血红蛋白和亚硝酸盐测定的干扰。
随机尿液标本纳入本研究。进行可比性、准确性和抗坏血酸干扰测试。
结果显示，在抗坏血酸阴性的样本中，两种试纸条对所有参数的一致性几乎完美。对于胆红素、蛋白质、亚硝酸盐和血红蛋白，抗坏血酸阳性样本中的一致性不可接受。两种试纸条上血红蛋白和白细胞酯酶的准确性均不可接受。抗坏血酸干扰检查表明，不同试纸条的干扰强度不同。抗坏血酸在不同浓度下会干扰葡萄糖、血红蛋白、亚硝酸盐和胆红素，导致假阴性结果。
所得结果表明，为了确定尿液分析的目的，有必要确定所用试纸条的诊断准确性。选择带有抗坏血酸指示剂的试纸条并独立于制造商声明检查抗坏血酸对试纸条分析物的影响非常重要。
