利用 CRISPR/Cas9 基因组编辑在小鼠中生成新型 Tau 基因敲除（tauΔex1）品系。
Generation of a New Tau Knockout (tauΔex1) Line Using CRISPR/Cas9 Genome Editing in Mice.
机构信息
Dementia Research Unit, School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia.
Transgenic Animal Unit, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, NSW, Australia.
出版信息
J Alzheimers Dis. 2018;62(2):571-578. doi: 10.3233/JAD-171058.
Alzheimer's disease and other dementias present with tau pathology. Several mouse lines with knockout of the tau-encoding Mapt gene have been reported, yet findings often differed between lines and sites. Here, we report a new tau knockout strain (tauΔex1), generated by CRISPR/Cas9-mediated genome editing of intron -1/exon 1 of Mapt in C57Bl/6J mice. TauΔex1 mice had no overt phenotype, but, in line with previous models, they showed a significantly reduced susceptibility to excitotoxic seizures, with normal memory formation in young mice. This new in vivo resource will be made freely available to the research community.
阿尔茨海默病和其他类型的痴呆症都伴随着 Tau 病理学。已经有报道称，几种敲除 Tau 编码基因 Mapt 的小鼠品系存在，但不同品系和实验地点的结果往往存在差异。在这里，我们报告了一种新的 Tau 敲除品系（tauΔex1），该品系是通过 C57Bl/6J 小鼠 Mapt 基因的内含子 1/外显子 1 的 CRISPR/Cas9 介导的基因组编辑产生的。tauΔex1 小鼠没有明显的表型，但与以前的模型一样，它们对兴奋性癫痫发作的敏感性显著降低，年轻小鼠的记忆形成正常。这一新的体内资源将免费提供给研究界。
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