Krieg Sarah, Lüscher Bernhard, Vervoorts Jörg, Dohmen Marc
Institute of Biochemistry and Molecular Biology, Medical School, RWTH Aachen University, Aachen, Germany.
Methods Mol Biol. 2018;1732:373-391. doi: 10.1007/978-1-4939-7598-3_24.
AMPK is an energy-sensing kinase and is required for the induction and progression of the autophagy process. In this chapter, we describe experimental approaches to study the steady state and flux of autophagy in response to AMPK activation. For this purpose, we provide detailed protocols for the measurement of general as well as AMPK-specific autophagy markers by immunoblot and immunofluorescence analysis.
AMPK是一种能量感应激酶，在自噬过程的诱导和进展中发挥作用。在本章中，我们描述了研究自噬稳态和通量以响应AMPK激活的实验方法。为此，我们提供了通过免疫印迹和免疫荧光分析来测量一般以及AMPK特异性自噬标志物的详细方案。
