Martinez-Sanchez Aida, Nguyen-Tu Marie-Sophie, Leclerc Isabelle, Rutter Guy A
Section of Cell Biology and Functional Genomics, Division of Diabetes, Endocrinology and Metabolism, Imperial College London, London, UK.
Methods Mol Biol. 2018;1732:413-431. doi: 10.1007/978-1-4939-7598-3_26.
The role of the energy sensor AMPK-activated protein kinase (AMPK) in the insulin-secreting β-cell remains unclear and a subject of intense research. With this chapter, we aim to provide a detailed description of the methods that our group routinely applies to the study of AMPK function in mouse and human pancreatic islets. Thus, we provide detailed protocols to isolate and/or culture mouse and human islets, to modulate and measure AMPK activity in isolated islets, and to evaluate its impact on islet function.
能量传感器腺苷酸活化蛋白激酶（AMPK）在胰岛素分泌β细胞中的作用仍不清楚，是深入研究的一个课题。在本章中，我们旨在详细描述我们团队常规用于研究AMPK在小鼠和人类胰岛中功能的方法。因此，我们提供了分离和/或培养小鼠和人类胰岛、调节和测量分离胰岛中AMPK活性以及评估其对胰岛功能影响的详细方案。
