秀丽隐杆线虫AMPK缺陷型在急性饥饿后的跨代表型分析

Analysis of Transgenerational Phenotypes Following Acute Starvation in AMPK-Deficient C. elegans.

作者信息

Demoinet Emilie, Roy Richard

机构信息

Department of Biology, McGill University, Montreal, QC, Canada.

Institute of Biology Valrose (iBV), CNRS, INSERM, University Côte d'Azur, Nice, France.

出版信息

Methods Mol Biol. 2018;1732:565-579. doi: 10.1007/978-1-4939-7598-3_36.

DOI:10.1007/978-1-4939-7598-3_36

PMID:29480500

Abstract

Environmental variation experienced early in life can result in long-term reproductive consequences. We have recently identified an important role for AMPK in the prevention of transgenerational defects following starvation of L1 stage larvae in C. elegans. Here we describe a means of analyzing these transgenerational defects following a single exposure to energy stress during early larval development. We also provide methods to quantify the histone modifications that are affected by this stress, along with the resulting reproductive defects that arise in later generations.

摘要

生命早期经历的环境变化会导致长期的生殖后果。我们最近发现，AMPK在预防秀丽隐杆线虫L1期幼虫饥饿后的跨代缺陷方面发挥着重要作用。在此，我们描述了一种在幼虫早期发育期间单次暴露于能量应激后分析这些跨代缺陷的方法。我们还提供了量化受这种应激影响的组蛋白修饰的方法，以及后代中出现的由此产生的生殖缺陷的方法。