Sugawara I, Kitagawa H, Inagaki H, De Ley M, Fukuda A
Microbiol Immunol. 1986;30(10):1049-59. doi: 10.1111/j.1348-0421.1986.tb03034.x.
We explored the population of Interferon-gamma (IFN-gamma) containing cells in order to clarify their cell surface phenotypic markers. Here we define gamma-IFN containing cells as gamma-IFN plaque forming cells (PFC). By this method, it was found that IFN-gamma containing cells consist of two cell fractions, i.e., OKT3+, OKT4+, and OKT8- cells and OKM1+ cells. Effective IFN-gamma production seems to require participation of plastic-adherent cells (presumably monocytes), while the addition of cyclosporin A (CyA) almost completely blocked generation of human IFN-gamma. To characterize Con A-stimulated IFN-gamma containing cells, we performed two-color flow cytometry using FACS IV. Most of the IFN-gamma containing cells have surface phenotypic markers for Leu3, Leu8, Leu15, HLA-DR, and IL-2 receptors, but most lack markers for Leu2 and Leu7. Interestingly, most of Leu3+ and IL-2 receptor+ cells belong to the dimly illuminating cell fractions of the IFN-gamma containing cell population. Our results indicate that IFN-gamma containing cells are heterogeneous with respect to surface phenotypic markers but the predominant IFN-gamma containing cell type is the helper T cell (OKT4+). Lastly, OK432, glycyrrhizin, and CCA (lobenzarit disodium) increase the number of IFN-gamma containing cells and are thought to be immunomodulators.
我们探究了含γ干扰素（IFN-γ）细胞的群体，以阐明其细胞表面表型标志物。在此，我们将含γ干扰素的细胞定义为γ干扰素空斑形成细胞（PFC）。通过这种方法，发现含IFN-γ的细胞由两个细胞亚群组成，即OKT3 +、OKT4 +和OKT8 -细胞以及OKM1 +细胞。有效的IFN-γ产生似乎需要黏附于塑料的细胞（可能是单核细胞）参与，而添加环孢素A（CyA）几乎完全阻断了人IFN-γ的产生。为了表征伴刀豆球蛋白A刺激的含IFN-γ细胞，我们使用FACS IV进行了双色流式细胞术检测。大多数含IFN-γ的细胞具有Leu3、Leu8、Leu15、HLA-DR和IL-2受体的表面表型标志物，但大多数缺乏Leu2和Leu7的标志物。有趣的是，大多数Leu3 +和IL-2受体 +细胞属于含IFN-γ细胞群体中荧光较弱的细胞亚群。我们的结果表明，含IFN-γ的细胞在表面表型标志物方面具有异质性，但主要的含IFN-γ细胞类型是辅助性T细胞（OKT4 +）。最后，OK432、甘草甜素和CCA（氯苯扎利二钠）可增加含IFN-γ细胞的数量，被认为是免疫调节剂。
