Kobayashi Takashi, Sato Yu, Nishiumi Shin, Yagi Yosuke, Sakai Arata, Shiomi Hideyuki, Masuda Atsuhiro, Okaya Shinobu, Kutsumi Hiromu, Yoshida Masaru, Honda Kazufumi
Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Japan.
Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Japan.
Biochem Biophys Res Commun. 2018 Mar 11;497(3):903-907. doi: 10.1016/j.bbrc.2018.02.170. Epub 2018 Feb 23.
Recently, apolipoprotein A2 (apoA2) isoforms have been reported as candidate serum/plasma biomarkers of pancreatic cancer. However, the distribution of apoA2 isoforms in patients with autoimmune pancreatitis (AIP) has not been investigated yet. In this study, we evaluated the distribution of serum apoA2 isoforms; i.e., homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, and homodimer apoA2-AT/AT, in AIP patients and healthy volunteers (HV) using enzyme-linked immunosorbent assays, and the clinical characteristics and serum levels of each apoA2 isoform in 32 AIP patients and 38 HV were investigated. The calculated apoA2-ATQ/AT levels of the AIP patients were significantly lower than those of the HV, which agreed with results obtained for patients with pancreatic cancer. Interestingly, most of the AIP patients exhibited high levels of apoA2-ATQ along with low levels of apoA2-AT, indicating that the processing of the C-terminal regions of apoA2 dimer was inhibited in the AIP patients. This specific distribution of serum apoA2 isoforms might provide important information about the disease states of AIP patients and aid the differential diagnosis of AIP versus pancreatic cancer.
最近，载脂蛋白A2（apoA2）亚型已被报道为胰腺癌的候选血清/血浆生物标志物。然而，apoA2亚型在自身免疫性胰腺炎（AIP）患者中的分布尚未得到研究。在本研究中，我们使用酶联免疫吸附测定法评估了AIP患者和健康志愿者（HV）血清中apoA2亚型的分布，即同型二聚体apoA2 - ATQ/ATQ、异型二聚体apoA2 - ATQ/AT和同型二聚体apoA2 - AT/AT，并对32例AIP患者和38例HV的每种apoA2亚型的临床特征和血清水平进行了研究。AIP患者计算得出的apoA2 - ATQ/AT水平显著低于HV，这与胰腺癌患者的结果一致。有趣的是，大多数AIP患者表现出高水平的apoA2 - ATQ和低水平的apoA2 - AT，这表明apoA2二聚体C末端区域的加工在AIP患者中受到抑制。血清apoA2亚型的这种特定分布可能为AIP患者的疾病状态提供重要信息，并有助于AIP与胰腺癌的鉴别诊断。
