State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd, Shanghai 200436, China; School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China.
School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China.
Food Chem Toxicol. 2018 Sep;119:296-301. doi: 10.1016/j.fct.2018.02.052. Epub 2018 Feb 24.
As one of low-digestible proteins, tartary buckwheat protein (BWP) revealed a cholesterol-lowering activity. The relationship between the prevention of BWP on dyslipidemia and changes in the numbers of gut microbiota was investigated. The male C57BL/6 mice were separately fed on normal diet, high-fat diet (HFD) with casein, and HFD with BWP extract for 6 weeks. Quantitative PCR assay was applied to quantify the microbiota composition in feces. The levels of plasma total cholesterol (TC) and triglyceride (TG) in the mice fed on HFD with BWP were significantly lower than those on HFD with casein. BWP promoted the growth of Lactobacillus, Bifidobacterium and Enterococcus and inhibited the growth of Escherichia coli in HFD-fed mice. Moreover, Bifidobacterium population was closely related to contents of plasma lipids. Further, BWP significantly decreased the levels of plasma inflammation factors as induced by HFD, including lipopolysaccharide, tumor necrosis factor α and interleukin 6. BWP significantly increased the excretion of total bile acids and short-chain fatty acids in feces. In conlusion, BWP benefited cholesterol metabolism, which could be attributed to regulating composition of gut microbiota.
作为一种低消化性蛋白质，苦荞蛋白（BWP）具有降低胆固醇的活性。本研究旨在探讨 BWP 预防血脂异常与肠道微生物菌群数量变化的关系。雄性 C57BL/6 小鼠分别用正常饮食、酪蛋白高脂饮食（HFD）和 BWP 提取物 HFD 喂养 6 周。采用定量 PCR 法检测粪便中微生物组成。与 HFD 喂养的酪蛋白组相比，BWP 喂养的 HFD 组小鼠血浆总胆固醇（TC）和甘油三酯（TG）水平显著降低。BWP 促进了 HFD 喂养小鼠中乳杆菌、双歧杆菌和肠球菌的生长，抑制了大肠杆菌的生长。此外，双歧杆菌种群与血浆脂质含量密切相关。此外，BWP 还显著降低了 HFD 诱导的血浆炎症因子（如脂多糖、肿瘤坏死因子-α和白细胞介素 6）的水平。BWP 还显著增加了粪便中总胆汁酸和短链脂肪酸的排泄。综上所述，BWP 有益于胆固醇代谢，这可能归因于其对肠道微生物菌群组成的调节。
