推桥弓后粥样硬化颈动脉的单轴拉伸特性：一项基于颈动脉粥样硬化动物模型的安全性研究
Uniaxial Tensile Properties of Atherosclerotic Carotid Artery After Mobilization of Pushing on Qiao-Gong: A Safety Study Using an Animal Model of Carotid Atherosclerosis.
作者信息
Qi Ji, Zhang Shaoqun, Zhang Lei, Ping Ruiyue, Ping Kaike, Ye Da, Shen Honggui, Chen Yili, Li Yikai
机构信息
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China.
Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, Sichuan Province, China.
出版信息
J Manipulative Physiol Ther. 2018 Feb;41(2):164-173. doi: 10.1016/j.jmpt.2017.12.002.
OBJECTIVES
This study aimed to preliminarily explore the effects of the soft tissue mobilization of pushing on Qiao-Gong (MPQ) on biomechanical properties of the carotid artery using an animal model of carotid atherosclerosis (CAS).
METHODS
Fifty rabbits were randomly divided into 4 groups: animals with CAS treated with MPQ (CAS-MPQ [n = 15]); animals with CAS treated without MPQ (CAS [n = 15]); normal animals treated with MPQ (normal-MPQ [n = 10]); and a blank control group (n = 10). The MPQ procedure consisted of soft tissue mobilization of the Qiao-Gong acupoint on the front edge of the sternocleidomastoid muscle applied from top to bottom, by flat pushing with the thumb repeatedly for 20 times. Disease in the CAS models was induced by carotid artery balloon injury combined with a high-fat diet for 12 weeks. At the end of modeling, carotid color Doppler ultrasonography examination was performed to confirm which animal models were successfully induced with CAS, excluding model rabbits without typical CAS at the same time. Then, MPQ was applied on rabbits in the CAS-MPQ and the normal-MPQ groups for 3 weeks. By contrast, rabbits in the other 2 groups were fed normally without MPQ. Uniaxial failure tests were later performed on carotid arteries in all 4 groups, and at the end of the study, a 2-way factorial analysis of variance of the results was conducted.
RESULTS
(1) At the end of modeling, 10 rabbits in the CAS-MPQ group and 9 in the CAS group were included with typical carotid atherosclerotic characteristics. (2) Young's elastic modulus of the rabbit carotid artery increased more significantly in the CAS-MPQ group than the CAS group. (3) Compared with normal rabbit carotid arteries, atherosclerotic carotid arteries had lower levels of ultimate stress and ultimate strain but higher levels of ultimate load.
CONCLUSIONS
The uniaxial tensile mechanical properties of the rabbit atherosclerotic carotid artery were impaired after MPQ.
目的
本研究旨在利用颈动脉粥样硬化（CAS）动物模型初步探讨推桥弓（MPQ）软组织松动术对颈动脉生物力学特性的影响。
方法
50只家兔随机分为4组：接受MPQ治疗的CAS动物（CAS-MPQ组[n = 15]）；未接受MPQ治疗的CAS动物（CAS组[n = 15]）；接受MPQ治疗的正常动物（正常-MPQ组[n = 10]）；以及空白对照组（n = 10）。MPQ操作包括对胸锁乳突肌前缘桥弓穴位进行软组织松动，由上至下用拇指平推，反复20次。通过颈动脉球囊损伤联合高脂饮食12周诱导CAS模型发病。建模结束时，进行颈动脉彩色多普勒超声检查，确认哪些动物模型成功诱导出CAS，同时排除无典型CAS的模型兔。然后，对CAS-MPQ组和正常-MPQ组的家兔进行3周的MPQ治疗。相比之下，其他2组家兔正常饲养，不进行MPQ治疗。随后对所有4组的颈动脉进行单轴破坏试验，研究结束时，对结果进行双向析因方差分析。
结果
（1）建模结束时，CAS-MPQ组有10只家兔、CAS组有9只家兔具有典型的颈动脉粥样硬化特征。（2）CAS-MPQ组家兔颈动脉的杨氏弹性模量比CAS组增加更显著。（3）与正常家兔颈动脉相比，动脉粥样硬化颈动脉的极限应力和极限应变水平较低，但极限负荷水平较高。
结论
MPQ后家兔动脉粥样硬化颈动脉的单轴拉伸力学性能受损。
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