Ding Yuanyuan, Yao Peng, Hong Tao, Han Zhenkai, Zhao Baisong, Chen Weimin, Zhou Guangyu
Department of Pain Management, Shengjing Hospital of China Medical University, Shenyang, China.
Department of Anesthesiology, Guangzhou Women and Children's Medical Center, Guangzhou, China.
Oncotarget. 2018 Jan 3;9(7):7513-7521. doi: 10.18632/oncotarget.23867. eCollection 2018 Jan 26.
Neuropathic pain is pain caused by injury or dysfunction in the central and/or peripheral nervous system. Neuropathic pain has a high incidence with a complex mechanism, but effective treatment remains elusive. Hyperbaric oxygen (HBO) therapy has been widely used in the treatment of a variety of neurological diseases. The current study used a rat model of neuropathic pain induced by chronic constriction injury (CCI) of the sciatic nerve. We observed the effects of early use of 2.5 absolute atmosphere (ATA) HBO on neuropathic pain-related behaviors and the expression of nitric oxide synthase (NOS) isoforms in the spinal dorsal horn. In the CCI group, mechanical withdrawal threshold (MWT) was decreased, Thermal withdrawal latency (TWL) was shortened, and mRNA and protein levels of iNOS and nNOS were significantly increased compared to the sham group. MWT was increased, TWL was enhanced, and iNOS and nNOS levels were significantly decreased in the HBO group compared to the CCI group. There was no change in eNOS levels across all groups. HBO treatment at early stages can improve hyperalgesia.
神经病理性疼痛是由中枢和/或外周神经系统损伤或功能障碍引起的疼痛。神经病理性疼痛发病率高，机制复杂，但有效的治疗方法仍然难以捉摸。高压氧（HBO）疗法已广泛应用于多种神经系统疾病的治疗。本研究采用坐骨神经慢性压迫损伤（CCI）诱导的神经病理性疼痛大鼠模型。我们观察了早期使用2.5绝对大气压（ATA）HBO对神经病理性疼痛相关行为以及脊髓背角一氧化氮合酶（NOS）亚型表达的影响。与假手术组相比，CCI组的机械缩足阈值（MWT）降低，热缩足潜伏期（TWL）缩短，诱导型NOS（iNOS）和神经元型NOS（nNOS）的mRNA和蛋白水平显著升高。与CCI组相比，HBO组的MWT增加，TWL延长，iNOS和nNOS水平显著降低。各组内皮型NOS（eNOS）水平无变化。早期HBO治疗可改善痛觉过敏。
