Department of Pharmacology and Toxicology, College of Pharmacy, Taif University, Taif, Makkah 21974, Kingdom of Saudi Arabia.
Department of Clinical Pharmacy, College of Pharmacy, Taif University, Taif, Makkah 21974, Kingdom of Saudi Arabia.
Mol Med Rep. 2018 Apr;17(4):6059-6067. doi: 10.3892/mmr.2018.8607. Epub 2018 Feb 15.
Huernia Sp. Nov. aff. Boleana, Apocynaceae, grows in the high mountains of southwest Saudi Arabia and is widely used as a remedy for the treatment of diabetes. The present study investigated the anti‑inflammatory, wound healing and inhibitory effects on migration of Huernia Sp. Nov. aff. Boleana. The anti‑inflammatory effect was assessed in mice using formalin‑induced edema. Wound healing effects were assessed in rats using a circular excision wound model. An in vitro 'scratch' test was used to investigate the inhibitory effects on melanoma cell (B16‑F10) migration. The anti‑inflammatory effects of total extract, hexane and chloroform fractions were greater or equal to indomethacin (control). The relatively non‑polar fractions (hexane and chloroform) exhibited higher anti‑inflammatory activities compared with the aqueous fraction. The percentage of wound contraction among animals treated with the plant extract was higher compared with the control; however, this difference was not statistically significant (P>0.05). The total plant extract increased wound healing by inhibiting the inflammatory response, promoting angiogenesis, and significantly promoting the proliferation of fibroblasts, particularly on days 7 and 14 post‑wounding. Furthermore, the plant extract promoted wound repair via the enhancement of collagen synthesis, and complete epithelization with well‑formed and differentiated epithelial tissues. The in vitro 'scratch' test indicated the inhibitory effects of this plant on melanoma cell migration in a dose‑dependent manner. The present study indicated that Huernia Sp. Nov. aff. Boleana may have potential as an anti‑inflammatory, wound-healing and migration-inhibiting ethno medicine.
胡尔尼亚属新种。附属博莱纳，夹竹桃科，生长在沙特阿拉伯西南部的高山上，被广泛用作治疗糖尿病的药物。本研究调查了胡尔尼亚属新种。附属博莱纳的抗炎、愈合伤口和抑制迁移的作用。使用福马林诱导的水肿在小鼠中评估抗炎作用。使用圆形切除伤口模型在大鼠中评估伤口愈合作用。体外“划痕”试验用于研究对黑素瘤细胞（B16-F10）迁移的抑制作用。总提取物、己烷和氯仿级分的抗炎作用大于或等于吲哚美辛（对照）。与水相部分相比，相对非极性部分（己烷和氯仿）表现出更高的抗炎活性。与对照相比，用植物提取物处理的动物中的伤口收缩百分比更高;然而，这种差异没有统计学意义（P>0.05）。总植物提取物通过抑制炎症反应、促进血管生成以及显著促进成纤维细胞增殖来增加伤口愈合，特别是在创伤后第 7 天和第 14 天。此外，该植物提取物通过增强胶原蛋白合成和形成具有良好分化的上皮组织的完全上皮化来促进伤口修复。体外“划痕”试验表明，这种植物对黑素瘤细胞迁移具有剂量依赖性的抑制作用。本研究表明，Huernia Sp. Nov. aff. Boleana 可能具有作为抗炎、伤口愈合和抑制迁移的民族医学的潜力。
