Department of Implantology, School of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.
Mol Med Rep. 2018 Apr;17(4):6084-6092. doi: 10.3892/mmr.2018.8628. Epub 2018 Feb 22.
Bone remodeling is under the control of various signals and systems in the body, including the nervous system. Semaphorin (Sema) 3A is a chemorepellent protein which regulates bone mass. Schwann cells, having a pivotal role following nerve injury, interact with Sema3A under numerous circumstances. The present study established a co‑culture system of MG63 and Schwann cells to investigate the role of the interaction between Sema3A and Schwann cells in osteogenesis. The results from the alkaline phosphatase assay, calcium nodule staining and the analysis of the osteogenic gene expression revealed that Sema3A inhibits osteogenic differentiation of MG63 cells in single‑cell culture and promotes osteogenic differentiation of MG63 cells in co‑culture with Schwann cells, in a concentration‑dependent manner. These findings suggest that the presence of Schwann cells induces Sema3A‑associated osteogenic differentiation in bone cells, and also reveals the pivotal role of Sema3A as a regulator in the skeletal and nervous systems, thus contributing to a better understanding of the interaction between these systems.
骨重建受体内各种信号和系统的控制，包括神经系统。Sema3A 是一种趋化抑制蛋白，可调节骨量。雪旺细胞在神经损伤后起着关键作用，在许多情况下与 Sema3A 相互作用。本研究建立了 MG63 和雪旺细胞的共培养体系，以研究 Sema3A 与雪旺细胞之间的相互作用在成骨中的作用。碱性磷酸酶测定、钙结节染色和成骨基因表达分析的结果表明，Sema3A 抑制 MG63 细胞在单细胞培养中的成骨分化，并以浓度依赖的方式促进 MG63 细胞与雪旺细胞共培养中的成骨分化。这些发现表明雪旺细胞的存在诱导了骨细胞中与 Sema3A 相关的成骨分化，也揭示了 Sema3A 作为骨骼和神经系统中调节剂的关键作用，从而有助于更好地理解这些系统之间的相互作用。
