Kumar S, Gupta E, Kaushik S, Kumar Srivastava V, Mehta S K, Jyoti A
Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur, India.
Department of General Medicine, SMS Medical College & Attached Hospitals, Jaipur, India.
Scand J Immunol. 2018 Apr;87(4):e12653. doi: 10.1111/sji.12653.
Sepsis is a condition caused by infection followed by unregulated inflammatory response which may lead to the organ dysfunction. During such condition, over-production of oxidants is one of the factors which contribute cellular toxicity and ultimately organ failure and mortality. Antioxidants having free radicals scavenging activity exert protective role in various diseases. This study has been designed to evaluate the levels of oxidative and antioxidative activity in sepsis patients and their correlation with the severity of the sepsis. A total of 100 sepsis patients and 50 healthy controls subjects were enrolled in this study from the period October 2016 to June 2017. The investigation included measurements of oxidative enzyme, myeloperoxidase (MPO), antioxidant enzymes including superoxide dismutase activity (SOD) and catalase activity (CAT) and cytokines (TNF-α, IL-8 and IFN-γ). Furthermore, the level of these activities was correlated with severity of sepsis. Augmented levels of oxidants were found in sepsis as demonstrated by DMPO nitrone adduct formation and plasma MPO level activity (1.37 ± 0.51 in sepsis vs 0.405 ± 0.16 in control subjects). Cytokines were also found to be increased in sepsis patients. However, plasma SOD and CAT activities were significantly attenuated (P < .001) in the sepsis patients compared with controls subjects. Moreover, inverse relation between antioxidant enzymes (SOD and CAT) and organ failure assessment (SOFA), physiological score (APACHE II), organ toxicity specific markers have been observed as demonstrated by Pearson's correlation coefficient. This study suggests that imbalance between oxidant and antioxidant plays key role in the severity of sepsis.
脓毒症是一种由感染引发的病症，随后会出现不受控制的炎症反应，这可能导致器官功能障碍。在这种情况下，氧化剂的过度产生是导致细胞毒性并最终导致器官衰竭和死亡的因素之一。具有自由基清除活性的抗氧化剂在各种疾病中发挥保护作用。本研究旨在评估脓毒症患者的氧化和抗氧化活性水平及其与脓毒症严重程度的相关性。2016年10月至2017年6月期间，本研究共纳入了100例脓毒症患者和50例健康对照者。调查包括测量氧化酶、髓过氧化物酶（MPO）、抗氧化酶，包括超氧化物歧化酶活性（SOD）和过氧化氢酶活性（CAT）以及细胞因子（TNF-α、IL-8和IFN-γ）。此外，这些活性水平与脓毒症的严重程度相关。如DMPO硝酮加合物形成和血浆MPO水平活性所示，脓毒症患者体内氧化剂水平升高（脓毒症患者为1.37±0.51，对照组为0.405±0.16）。脓毒症患者的细胞因子也有所增加。然而，与对照组相比，脓毒症患者的血浆SOD和CAT活性显著降低（P<0.001）。此外，通过Pearson相关系数显示，抗氧化酶（SOD和CAT）与器官衰竭评估（SOFA）、生理评分（APACHE II）、器官毒性特异性标志物之间存在负相关。本研究表明，氧化剂和抗氧化剂之间的失衡在脓毒症的严重程度中起关键作用。
