Hirad Abdurahman H, Ahmad Javed, Alkhedhairy Abdulaziz A, Bahkali Ali H, Khan Shams T
Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
APMIS. 2018 Mar;126(3):215-226. doi: 10.1111/apm.12802.
Customary consumption of unpasteurized milk by the population in the central Najed region of Saudi Arabia may pose a health risk. Therefore, 80 camel milk samples were collected aseptically from seven different stations of Riyadh region. The biochemical and microbiological properties of these milk samples were determined. Nutrient agar and brain heart infusion agar were used to determine mesophilic aerobic counts (MACs). The MAC in each mL of milk varied from 60 to 16 × 10 CFU/mL on nutrient agar. Based on the colony morphology, 176 colonies were collected from different samples, and these isolates were de-replicated into 80 unique isolates using rep-PCR analysis. Surprisingly, the 16S rRNA sequence analysis of these strains revealed that more than one-third of the collected milk samples contained strains that share maximum sequence similarities with well-known pathogens, such as Brucella, Bacillus anthracis, Listeria monocytogenes, and MRSA. Furthermore, many strains exhibit 16S rRNA gene similarity with opportunistic pathogens such as Citrobacter freundii and Kytococcus schroeteri. Many strains exhibit β-hemolytic activity and resistant to six different antibiotics. Our study suggested that consumption of raw camel milk from this region constitutes a great health risk.
沙特阿拉伯纳杰德中部地区居民习惯饮用未经巴氏杀菌的牛奶，这可能会带来健康风险。因此，从利雅得地区的七个不同站点无菌采集了80份骆驼奶样本。测定了这些牛奶样本的生化和微生物特性。使用营养琼脂和脑心浸液琼脂来测定嗜温需氧菌数（MACs）。在营养琼脂上，每毫升牛奶中的MACs在60至16×10⁶CFU/mL之间。根据菌落形态，从不同样本中收集了176个菌落，并使用rep-PCR分析将这些分离株去重复为80个独特的分离株。令人惊讶的是，对这些菌株的16S rRNA序列分析表明，超过三分之一的采集牛奶样本中所含菌株与布鲁氏菌、炭疽芽孢杆菌、单核细胞增生李斯特菌和耐甲氧西林金黄色葡萄球菌等知名病原体具有最大序列相似性。此外，许多菌株与弗氏柠檬酸杆菌和施氏库克菌等机会性病原体表现出16S rRNA基因相似性。许多菌株表现出β-溶血活性并对六种不同抗生素具有抗性。我们的研究表明，饮用该地区的生骆驼奶会带来很大的健康风险。
