Orlacchio A, Emiliani C, Rambotti P, Pioda G B, Davis S
Biochem Med Metab Biol. 1986 Dec;36(3):283-92. doi: 10.1016/0885-4505(86)90137-4.
beta-Hexosaminidase (EC 3.2.1.20; Hex) activity and isoenzyme characteristics were analyzed in human normal and leukemic leukocytes. Unseparated CLL and CML cells had a specific activity that was lower, whereas ALL and AML blasts had a higher specific activity than normal lymphocytes and granulocytes. CLL B-cells had a lower specific activity compared with that in normal non-T-lymphocytes; CLL T-cells and normal T-cells had similar activity. Isoenzyme separation was performed by chromatofocusing on PBE-94 coupled with an automated enzyme assay. When using a single linear pH elution gradient, normal leukocytes and all leukemia cells contained two forms of isoenzyme (B and A). When a double pH elution gradient was performed, an extra distinct form of Hex (I) was recorded. Hex I was present in small amounts in normal granulocytes and PHA-stimulated normal lymphocytes; isoenzyme I was found in high amounts in all leukemias tested. The activity ratios I/B and I/A, as well as the I isoenzyme profile, may facilitate differentiation between normal and leukemic cells and between lymphoblastic and myeloblastic leukemias.
在人正常白细胞和白血病白细胞中分析了β-己糖胺酶（EC 3.2.1.20；Hex）活性及同工酶特征。未经分离的慢性淋巴细胞白血病（CLL）和慢性粒细胞白血病（CML）细胞的比活性较低，而急性淋巴细胞白血病（ALL）和急性髓细胞白血病（AML）原始细胞的比活性高于正常淋巴细胞和粒细胞。与正常非T淋巴细胞相比，CLL B细胞的比活性较低；CLL T细胞和正常T细胞具有相似的活性。通过在PBE - 94上进行色谱聚焦结合自动酶测定法进行同工酶分离。当使用单一线性pH洗脱梯度时，正常白细胞和所有白血病细胞均含有两种形式的同工酶（B和A）。当进行双pH洗脱梯度时，记录到一种额外的独特形式的Hex（I）。Hex I在正常粒细胞和植物血凝素（PHA）刺激的正常淋巴细胞中少量存在；在所有测试的白血病中均发现大量的同工酶I。活性比I/B和I/A以及I同工酶谱可能有助于区分正常细胞和白血病细胞以及淋巴细胞白血病和髓细胞白血病。
