Orlacchio A, Maffei C, Emiliani C, Rambotti P, Davis S
Biochem Biophys Res Commun. 1984 Aug 16;122(3):966-73. doi: 10.1016/0006-291x(84)91186-0.
The beta-hexosaminidase (EC 3.2.1.30) isoenzymes were separated on the basis of their carbohydrate moieties by an affinity chromatography using immobilized phenylboronate. Normal lymphocytes and granulocytes contain two major forms of beta-hexosaminidase, acute lymphoblastic, acute myeloblastic, chronic lymphocytic and chronic myelocytic leukemic cells contain an extra, distinct isoenzyme of beta-hexosaminidase. This extra isoenzyme may be a marker for the leukemic conversion of hematopoietic tissue.
利用固定化苯硼酸通过亲和色谱法，根据β-己糖胺酶（EC 3.2.1.30）同工酶的碳水化合物部分对其进行分离。正常淋巴细胞和粒细胞含有两种主要形式的β-己糖胺酶，急性淋巴细胞性、急性髓细胞性、慢性淋巴细胞性和慢性髓细胞性白血病细胞含有一种额外的、独特的β-己糖胺酶同工酶。这种额外的同工酶可能是造血组织白血病转化的一个标志物。
