大鼠间皮瘤细胞在培养中的体外生长特性

In vitro growth characteristics of rat mesothelioma cells in culture.

作者信息

Jaurand M C, Renier A, Van der Meeren A, Magne L, Pinchon M C, Bignon J

出版信息

Biol Cell. 1986;57(3):249-56. doi: 10.1111/j.1768-322x.1986.tb00481.x.

DOI:10.1111/j.1768-322x.1986.tb00481.x

Abstract

The study reports morphological growth characteristics and chromosome analysis of neoplastic rat pleural mesothelial cells (RPMC) isolated from a mesothelioma-bearing rat. The pleural mesothelioma was induced by intrapleural injection of chrysotile fibers. Neoplastic RPMC were cultured by the standard methods used for normal RPMC. Neoplastic RPMC cultures had a population doubling time of 19 hr versus 30 hr for the normal cells. Plating efficiency in liquid medium was almost 100%. Cultures of neoplastic RPMC were anchorage-independent since 70% of the seeded cells formed colonies after one week; after the second week, colony size was enhanced but colony recovery was not. The serum dependence of neoplastic cells was less than that of the normal cells. 79 out of 100 metaphase cells analyzed had 41 to 43 chromosomes, and the modal number was 42 (38%). A large metacentric chromosome was observed in 77 of the 100 neoplastic metaphase cells analyzed, but not in any normal metaphase cells. In nude mice, the neoplastic RPMC were tumorigenic.

摘要

该研究报告了从一只患有间皮瘤的大鼠中分离出的肿瘤性大鼠胸膜间皮细胞（RPMC）的形态学生长特征和染色体分析。胸膜间皮瘤通过胸腔内注射温石棉纤维诱导产生。肿瘤性RPMC采用用于正常RPMC的标准方法进行培养。肿瘤性RPMC培养物的群体倍增时间为19小时，而正常细胞为30小时。在液体培养基中的接种效率几乎为100%。肿瘤性RPMC培养物不依赖贴壁生长，因为70%的接种细胞在一周后形成了集落；第二周后，集落大小增加但集落回收率未增加。肿瘤细胞对血清的依赖性低于正常细胞。在分析的100个中期细胞中，79个有41至43条染色体，众数为42条（38%）。在分析的100个肿瘤中期细胞中有77个观察到一条大的中着丝粒染色体，但在任何正常中期细胞中均未观察到。在裸鼠中，肿瘤性RPMC具有致瘤性。