National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, PR China.
National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, PR China; Key Laboratory of Agriculture Ministry for Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai, PR China.
Fish Shellfish Immunol. 2018 May;76:35-40. doi: 10.1016/j.fsi.2018.02.043. Epub 2018 Feb 24.
Crucian carp (Carassius auratus gibelio) is a popular food fish in Asia, and cyprinid herpesvirus 2 (CyHV-2) is the only known viral pathogen for crucian carp. Type I interferon genes are induced up on host cell recognition of viral nucleic acids and well recognized for their crucial roles in providing local or systemic protection against the viruses in various organisms. In a transcriptome analysis to uncover differentially expressed genes in crucian carp in response to CyHV-2 challenge, a partial interferon transcript was identified to be significantly up-regulated in the kidney of infected fish, which was named as crucian carp IFNc (ccIFNc). The complete ORF of ccIFNc was further determined by RACE technique, which spanned over 546 bp and encoded a polypeptide containing 182 amino acids. Phylogenetic analysis revealed that ccIFNc clustered with known type I IFN genes from other aquatic organisms. Quantitative RT-PCR analysis demonstrated that ccIFNc was constitutively expressed in all investigated tissues with a comparably higher expression level in spleen, gill, kidney, and muscle. Following challenge with CyHV-2, the transcriptional levels of ccIFNc were dramatically up-regulated in all of the tested tissues, especially in the spleen and gill with increased folds of 436 and 158, respectively. The intramuscular (i.m.) injection of a eukaryotic expression plasmid encoding ccIFNc (pEGFP-cIFNc) resulted in increased ccIFNc expression and reduced the mortality after the CyHV-2 challenge significantly. In summary, our data suggested that the ccIFNc belongs to the type I interferon family with a potential role in countering CyHV-2 infection in crucian carp.
鲫鱼（Carassius auratus gibelio）是亚洲一种受欢迎的食用鱼类，而鲤鱼疱疹病毒 2 型（CyHV-2）是唯一已知的鲫鱼病毒病原体。I 型干扰素基因在宿主细胞识别病毒核酸时被诱导表达，并且在各种生物体中为提供针对病毒的局部或系统保护方面发挥着重要作用。在一项针对鲫鱼对 CyHV-2 感染反应的转录组分析中，鉴定出一种干扰素转录本在感染鱼的肾脏中显著上调，将其命名为鲫鱼 IFNc（ccIFNc）。通过 RACE 技术进一步确定了 ccIFNc 的完整 ORF，该 ORF 跨越 546bp，编码含有 182 个氨基酸的多肽。系统进化分析表明，ccIFNc 与来自其他水生生物的已知 I 型 IFN 基因聚类。定量 RT-PCR 分析表明，ccIFNc 在所有研究的组织中均呈组成型表达，在脾脏、鳃、肾脏和肌肉中表达水平较高。用 CyHV-2 攻毒后，ccIFNc 在所有测试组织中的转录水平均显著上调，尤其是在脾脏和鳃中，分别增加了 436 倍和 158 倍。肌肉内（i.m.）注射编码 ccIFNc 的真核表达质粒（pEGFP-cIFNc）可导致 ccIFNc 表达增加，并显著降低 CyHV-2 攻毒后的死亡率。综上所述，我们的数据表明，ccIFNc 属于 I 型干扰素家族，在鲫鱼抵抗 CyHV-2 感染中具有潜在作用。
