Department of Ocean Technology, College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng, 224051, China.
Department of Ocean Technology, College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng, 224051, China; Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, 116023, China.
Fish Shellfish Immunol. 2020 Jul;102:13-19. doi: 10.1016/j.fsi.2020.03.065. Epub 2020 Apr 3.
Gibel carp (Carassius auratus gibelio) is an important commercial fish that has become one of the most cultured fishes in the region of Yangtze River in China. However, the fish faces increasing hazard due to cyprinid herpesvirus 2 (CyHV-2) infection, which has caused great economic losses. In this study, healthy gibel carp were intraperitoneally injected with different doses of poly I:C at 24 h before CyHV-2 challenge. Results showed that the mortality decreased and peak death time appeared later in the fish injected with poly I:C at a dose of 10 μg/g body weight. To explore what gene plays an important role after poly I:C treatment, the transcriptome analysis of the gibel carp spleen was further performed. Compared with the PBS group, 1286 differentially expressed genes (DEGs) were obtained in the poly I:C-treated fish, including 1006 up-regulated and 280 down-regulated DEGs. GO analysis revealed that the most enriched DEGs responded to "biological regulation", "regulation of cellular process" and "regulation of biological process". Meanwhile, KEGG enrichment analysis showed that the DEGs were mainly mapped on the immune pathways like "TNF signal pathway", "p53 signal pathway" and "JAK-STAT signal pathway", suggesting that these signal pathways may be responsible for the delayed peak of CyHV-2 infection in gibel carp after poly I:C treatment. Taken together, this study provides insights into the immune protection effect of poly I:C against CyHV-2 infection, as well as providing useful information for antiviral defense in gibel carp.
吉富罗非鱼（Carassius auratus gibelio）是一种重要的商业鱼类，已成为中国长江流域养殖最广泛的鱼类之一。然而，由于疱疹病毒 2 型（CyHV-2）感染，这种鱼面临着越来越大的威胁，这给鱼类养殖带来了巨大的经济损失。在本研究中，健康的吉富罗非鱼在 CyHV-2 攻毒前 24 小时通过腹腔注射不同剂量的聚肌胞苷酸（poly I:C）。结果表明，在注射 10μg/g 体重的 poly I:C 时，死亡率降低，峰值死亡时间延迟。为了探索 poly I:C 处理后哪些基因发挥重要作用，进一步对吉富罗非鱼脾脏进行了转录组分析。与 PBS 组相比，poly I:C 处理组的吉富罗非鱼获得了 1286 个差异表达基因（DEGs），其中 1006 个上调，280 个下调。GO 分析表明，最富集的 DEGs 对“生物调节”、“细胞过程调节”和“生物过程调节”有反应。同时，KEGG 富集分析表明，DEGs 主要映射在免疫途径上，如“TNF 信号通路”、“p53 信号通路”和“JAK-STAT 信号通路”，表明这些信号通路可能是导致 poly I:C 处理后吉富罗非鱼 CyHV-2 感染峰值延迟的原因。综上所述，本研究为 poly I:C 对 CyHV-2 感染的免疫保护作用提供了新的认识，并为吉富罗非鱼抗病毒防御提供了有用的信息。
