天冬氨酸功能化聚乙二醇化介孔二氧化硅纳米颗粒@氧化石墨烯杂化物作为一种用于体外药物递送的有效且可持续的纳米系统。
Aspartic acid functionalized PEGylated MSN@GO hybrid as an effective and sustainable nano-system for in-vitro drug delivery.
作者信息
Rahmatolahzadeh Reza, Hamadanian Masood, Ma'mani Leila, Shafiee Abbas
机构信息
Institute of Nanoscience and Nanotechnology, University of Kashan, Kashan, Iran.
Institute of Nanoscience and Nanotechnology, University of Kashan, Kashan, Iran; Department of Physical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran.
出版信息
Adv Med Sci. 2018 Sep;63(2):257-264. doi: 10.1016/j.advms.2018.01.003. Epub 2018 Mar 20.
PURPOSE
In this research, aspartic acid functionalized PEGylated mesoporous silica nanoparticlesgraphene oxide nanohybrid (As-PEGylated-MSN@GO) prepared as a pH-responsive drug carrier for the curcumin delivery. For better camouflage during blood circulation, poly(ethylene glycol) was decorated on the surface of MSN@GO nanohybrid.
MATERIALS AND METHODS
The nanocarrier was characterized by using X-ray powder diffraction (XRD), dynamic light scattering (DLS), UV-vis spectroscopy, thermal gravimetry analysis (TGA), FT-IR, SEM and TEM.
RESULTS
The size of modified MSN@GO was around 75.8 nm and 24% wt. of curcumin was loaded on the final nanohybrid. pHdecrement from 7.4 to 5.8 the release medium led to increase the cumulative amount of drug release from 54% to 98%.
CONCLUSIONS
As-functionalized MSN@GO had no cytotoxicity against human breast adenocarcinoma (MCF-7) and human mammary epithelial (MCF10A) as cancerous and normal cell lines, respectively. Whereas curcuminloaded nanohybrid showed excellent killing capability against MCF-7 cells.
目的
在本研究中，制备了天冬氨酸功能化的聚乙二醇化介孔二氧化硅纳米颗粒-氧化石墨烯纳米杂化物（As-PEGylated-MSN@GO）作为用于姜黄素递送的pH响应性药物载体。为了在血液循环过程中实现更好的伪装，在MSN@GO纳米杂化物表面修饰了聚乙二醇。
材料与方法
通过X射线粉末衍射（XRD）、动态光散射（DLS）、紫外可见光谱、热重分析（TGA）、傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）对纳米载体进行表征。
结果
修饰后的MSN@GO尺寸约为75.8 nm，最终的纳米杂化物负载了24%重量的姜黄素。将释放介质的pH从7.4降低到5.8导致药物释放的累积量从54%增加到98%。
结论
As功能化的MSN@GO对人乳腺腺癌（MCF-7）和人乳腺上皮细胞（MCF10A）分别作为癌细胞系和正常细胞系均无细胞毒性。而负载姜黄素的纳米杂化物对MCF-7细胞显示出优异的杀伤能力。
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