Liu Yang, Lin Jie, Zhai Shujuan, Sun Chunyi, Xu Changjun, Zhou Honglin, Liu Huijin
Department of Gynecology, the Second Affiliated Hospital of Kunming Medical University, Kunming, China.
Department of Oncology, the Second Affiliated Hospital of Kunming Medical University, Kunming, China.
Cell Physiol Biochem. 2018;45(4):1654-1662. doi: 10.1159/000487733. Epub 2018 Feb 21.
BACKGROUND/AIMS: Ovarian cancer is one of the most common malignancies with a high rate of mortality in women. However, current therapies for ovarian cancer treatment are ineffective. Therefore, novel target identification is an urgent requisite. The present study aimed to investigate the role of microRNA-214 (miR-214) in ovarian cancer.
The expression of miR-214, β-catenin, cyclin D1, c-myc, and TCF-1 at the transcriptional level was measured by real-time PCR, while that of β-catenin, Cyclin D1, and c-Myc at the protein level were detected by western blot. Colony formation assay and transwell assay were used to explore the invasion ability of the cancer cells. Cell cycle was measured by flow cytometry.
Real-time PCR showed that miR-214 expression in ovarian cancer cell lines was lower than that in the human normal ovarian epithelial cells, IOSE80. Furthermore, the low expression of miR-214 was correlated with high pathological grade. The rate of colony formation and invasion of miR-214 overexpression in SKOV-3 cells were weaker than that in control cells. Moreover, miR-214 overexpression led to the G0/G1 phase arrest. The expression of β-catenin, Cyclin D1, and c-Myc was suppressed by the overexpression of miR-214.
These results suggested that miR-214 may serve as a tumor suppressor of ovarian cancer by targeting the β-catenin pathway.
背景/目的：卵巢癌是女性中最常见的恶性肿瘤之一，死亡率很高。然而，目前用于治疗卵巢癌的疗法效果不佳。因此，识别新的靶点迫在眉睫。本研究旨在探讨微小RNA-214（miR-214）在卵巢癌中的作用。
通过实时聚合酶链反应（PCR）检测miR-214、β-连环蛋白、细胞周期蛋白D1、c-myc和TCF-1在转录水平的表达，同时通过蛋白质印迹法检测β-连环蛋白、细胞周期蛋白D1和c-Myc在蛋白质水平的表达。采用集落形成试验和Transwell试验探讨癌细胞的侵袭能力。通过流式细胞术检测细胞周期。
实时PCR显示，卵巢癌细胞系中miR-214的表达低于人正常卵巢上皮细胞IOSE80。此外，miR-214的低表达与高病理分级相关。SKOV-3细胞中miR-214过表达的集落形成率和侵袭率低于对照细胞。此外，miR-214过表达导致G0/G1期阻滞。miR-214过表达抑制了β-连环蛋白、细胞周期蛋白D1和c-Myc的表达。
这些结果表明，miR-214可能通过靶向β-连环蛋白通路发挥卵巢癌肿瘤抑制因子的作用。
