Department of Gynecology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Department of Geriatrics, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
IUBMB Life. 2020 Aug;72(8):1780-1786. doi: 10.1002/iub.2308. Epub 2020 Jun 5.
The present study aimed to investigate the molecular mechanism and the effect of Saponin from Tupistra chinensis Baker (STCB) on the proliferation and apoptosis of ovarian cancer cells. To investigate the inhibitory effect of STCB on the proliferation of ovarian cancer cells, SKOV3 cells were cultured and the methyl thiazolyl tetrazolium assay was used. Flow cytometry was also used to analyze the cell cycle distribution and apoptotic rate. Ki-67, cyclin D1, cleaved caspase-3, cleaved caspase-9, β-catenin, and c-Myc protein expressions were detected by western blot. Ovarian cancer cells were treated with STCB and Wnt pathway activator lithium chloride (LiCl). These methods were also used to determine the proliferation, cell cycle distribution, and apoptosis of ovarian cancer cells. In STCB-treated group, the proliferation inhibition and apoptosis rate, the proportion of G0-G1 phase, and the expression level of cleaved caspase-3 and 9 of ovarian cancer cells were significantly increased. Similarly, the expression of Ki-67, cyclin D1, β-catenin, and c-Myc were significantly decreased (p < .05). The results also showed that in STCB-LiCl-treated group, while the proliferation inhibition rate of ovarian cancer cells, the proportion of G0-G1 cells, the expression level of cleaved caspase-3 and 9, and the apoptosis rate (p < .05) were significantly decreased, the expression level of Ki-67, cyclin D1, β-catenin, and c-Myc was significantly increased. STCB induced G0-G1 phase arrest, inhibited cell proliferation, and promoted apoptosis of ovarian cancer cells by inhibiting Wnt/β-catenin pathway.
本研究旨在探讨朱顶红皂甙（STCB）对卵巢癌细胞增殖和凋亡的分子机制及作用。为了研究 STCB 对卵巢癌细胞增殖的抑制作用，培养 SKOV3 细胞，并采用噻唑蓝（MTT）比色法检测。流式细胞术分析细胞周期分布和凋亡率。Western blot 检测 Ki-67、cyclin D1、cleaved caspase-3、cleaved caspase-9、β-catenin 和 c-Myc 蛋白表达。用 STCB 和 Wnt 通路激活剂氯化锂（LiCl）处理卵巢癌细胞，同样采用这些方法来确定卵巢癌细胞的增殖、细胞周期分布和凋亡。在 STCB 处理组中，卵巢癌细胞的增殖抑制率和凋亡率、G0-G1 期比例以及 cleaved caspase-3 和 9 的表达水平均显著增加。同样，Ki-67、cyclin D1、β-catenin 和 c-Myc 的表达水平也显著降低（p<.05）。结果还表明，在 STCB-LiCl 处理组中，卵巢癌细胞的增殖抑制率、G0-G1 期细胞比例、cleaved caspase-3 和 9 的表达水平以及凋亡率（p<.05）均显著降低，而 Ki-67、cyclin D1、β-catenin 和 c-Myc 的表达水平显著增加。STCB 通过抑制 Wnt/β-catenin 通路诱导 G0-G1 期阻滞，抑制细胞增殖，促进卵巢癌细胞凋亡。
