Whisler R L, Newhouse Y G, Mortensen R F
Cell Immunol. 1986 Oct 15;102(2):287-98. doi: 10.1016/0008-8749(86)90422-3.
Utilizing a dual liquid/semisolid culture system with human B cells suspended in a semisolid matrix, T4 helper cells activated in the autologous mixed-lymphocyte reaction (AMLR) were much more effective than T8 suppressor cells in promoting B-cell colony formation in the presence of Staph protein A. C-reactive protein (CRP) inhibited the promotion of B-cell colony formation by autoactivated T cells and inhibition was observed only when CRP was present during the early AMLR. Coculture experiments revealed that CRP directly reduced T4 promotion of colony formation rather than inducing T8-mediated suppression of T4 cells. B cells preincubated with CRP responded normally to facilitative signals from autoactivated T cells; however, monocytes or T cells preincubated with CRP were reduced in AMLR promotion of colony formation. Other results indicated that CRP inhibition of colony formation during the AMLR was associated with considerable reductions in the proliferation of autoreactive T cells. These data demonstrate that CRP can directly reduce the ability of autoreactive T4 cells to expand the B-cell compartment and that CRP blocks T-cell activation events proximal to proliferation.
利用一种双液体/半固体培养系统，其中人类B细胞悬浮于半固体基质中，在葡萄球菌蛋白A存在的情况下，在自体混合淋巴细胞反应（AMLR）中被激活的T4辅助细胞在促进B细胞集落形成方面比T8抑制细胞有效得多。C反应蛋白（CRP）抑制了自激活T细胞对B细胞集落形成的促进作用，并且仅当CRP在早期AMLR期间存在时才观察到这种抑制作用。共培养实验表明，CRP直接降低了T4对集落形成的促进作用，而不是诱导T8介导的对T4细胞的抑制。预先用CRP孵育的B细胞对自激活T细胞的促进信号反应正常；然而，预先用CRP孵育的单核细胞或T细胞在AMLR中对集落形成的促进作用降低。其他结果表明，AMLR期间CRP对集落形成的抑制与自身反应性T细胞增殖的显著减少有关。这些数据表明，CRP可以直接降低自身反应性T4细胞扩展B细胞区室的能力，并且CRP阻断了增殖近端的T细胞激活事件。
