The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China.
College of Life Sciences, University of Chinese Academy of Sciences, 100049, Beijing, China.
Nat Commun. 2018 Feb 27;9(1):852. doi: 10.1038/s41467-018-03047-9.
The simultaneous improvement of grain quality and yield of cereal crops is a major challenge for modern agriculture. Here we show that a rice grain yield quantitative trait locus qLGY3 encodes a MADS-domain transcription factor OsMADS1, which acts as a key downstream effector of G-protein βγ dimers. The presence of an alternatively spliced protein OsMADS1 is shown to be associated with formation of long and slender grains, resulting in increases in both grain quality and yield potential of rice. The Gγ subunits GS3 and DEP1 interact directly with the conserved keratin-like domain of MADS transcription factors, function as cofactors to enhance OsMADS1 transcriptional activity and promote the co-operative transactivation of common target genes, thereby regulating grain size and shape. We also demonstrate that combining OsMADS1 allele with high-yield-associated dep1-1 and gs3 alleles represents an effective strategy for simultaneously improving both the productivity and end-use quality of rice.
同时提高谷物作物的粮食产量和品质是现代农业面临的主要挑战。在这里，我们表明，水稻粒重数量性状位点 qLGY3 编码一个 MADS 结构域转录因子 OsMADS1，它作为 G 蛋白βγ二聚体的关键下游效应因子起作用。存在一个选择性剪接的蛋白质 OsMADS1 与长而细的谷物的形成有关，导致水稻的产量和产量潜力都增加。Gγ亚基 GS3 和 DEP1 与 MADS 转录因子的保守角蛋白样结构域直接相互作用，作为辅因子起作用，增强 OsMADS1 的转录活性，并促进共同靶基因的协同转激活，从而调节谷物的大小和形状。我们还证明，将 OsMADS1 等位基因与高产相关的 dep1-1 和 gs3 等位基因相结合，是同时提高水稻生产力和最终用途品质的有效策略。
