Linninge Caroline, Roth Bodil, Erlanson-Albertsson Charlotte, Molin Göran, Toth Ervin, Ohlsson Bodil
Department of Food Technology, Engineering and Nutrition, Lund University, Lund S-223 63, Sweden.
Department of Internal Medicine, Skåne University Hospital, Lund University, Malmö S-205 02, Sweden.
World J Gastrointest Pathophysiol. 2018 Feb 15;9(1):18-27. doi: 10.4291/wjgp.v9.i1.18.
To compare gut bacterial diversity and amount of in colonic mucosa between patients with and without diverticular disease (DD).
Patients in a stable clinical condition with planned elective colonoscopy were included. Blood samples and colon mucosa biopsies were collected at the colonoscopy. Study questionnaires including questions about gastrointestinal symptoms were completed by the patients and physicians. DNA from mucosa samples was isolated and the amount of was estimated using PCR assay. Terminal restriction fragment length polymorphism was applied to assess microbial diversity. Diversity was estimated by calculations of richness (number of terminal restriction fragments) and Shannon-Wiener and Simpson's indices.
A total of 51 patients were included, 16 patients with DD [68 (62-76) years] and 35 controls [62 (40-74) years] without any diverticula. Patients with DD had significantly higher levels of than those without DD ( = 0.043), and there was an inverse relationship between the amount of and the Simpson's index (rs = -0.361, = 0.033) and the Shannon-Wiener index (rs = -0.299, = 0.081). The Simpson's index ( = 0.383), Shannon-Wiener index ( = 0.401) or number of restrictions fragments ( = 0.776) did not differ between DD and controls. The majority of patients experienced gastrointestinal symptoms, and 22 patients (43.1%) fulfilled the criteria for irritable bowel syndrome, with no difference between the groups ( = 0.212). Demography, socioeconomic status, lifestyle habits, inflammatory biomarkers, or symptoms were not related to the amount of or bacterial diversity.
Patients with DD had higher amount of in the colon mucosa compared to patients without diverticula.
比较憩室病（DD）患者与无憩室病患者结肠黏膜中的肠道细菌多样性及[此处原文缺失具体物质，无法准确翻译]数量。
纳入临床状况稳定且计划进行择期结肠镜检查的患者。在结肠镜检查时采集血样和结肠黏膜活检样本。患者和医生完成包括胃肠道症状问题的研究问卷。从黏膜样本中分离DNA，并使用聚合酶链反应（PCR）测定法估计[此处原文缺失具体物质，无法准确翻译]数量。应用末端限制性片段长度多态性来评估微生物多样性。通过计算丰富度（末端限制性片段数量）、香农 - 威纳指数和辛普森指数来估计多样性。
共纳入51例患者，16例DD患者[68（62 - 76）岁]和35例无任何憩室的对照者[62（40 - 74）岁]。DD患者的[此处原文缺失具体物质，无法准确翻译]水平显著高于无DD患者（= 0.043），且[此处原文缺失具体物质]数量与辛普森指数（rs = -0.361，= 0.033）和香农 - 威纳指数（rs = -0.299，= 0.081）呈负相关。DD患者与对照者之间的辛普森指数（= 0.383）、香农 - 威纳指数（= 0.401）或限制性片段数量（= 0.776）无差异。大多数患者有胃肠道症状，22例患者（43.1%）符合肠易激综合征标准，两组之间无差异（= 0.212）。人口统计学、社会经济状况、生活习惯、炎症生物标志物或症状与[此处原文缺失具体物质，无法准确翻译]数量或细菌多样性无关。
与无憩室患者相比，DD患者结肠黏膜中的[此处原文缺失具体物质，无法准确翻译]数量更高。
