Fiskin A M, Garrison R G, Figueroa M
Cytobios. 1986;48(194-195):185-93.
Hyphal cells of Neurospora crassa and Aspergillus nidulans, grown in Sabouraud glucose broth or in a defined medium with xanthine or its catabolites as the nitrogen source, contained single membrane-bound organelles cytochemically identified as microbodies. Modified Gomori procedures at the ultrastructural level revealed putative alkaline phosphatase activity sites in thin sections of cells of both species of fungi. Microbody membranes displayed electron opaque deposits (lead phosphate) which were absent in control preparations either lacking the substrate (beta-glycerophosphate) or the lead capture ion. Inhibition of this enzymic activity was achieved in parallel incubations fortified with the inhibitor levamisole. Cells grown in media containing limiting levels of inorganic phosphate revealed additional alkaline phosphatase activity at and along the nuclear membrane and endoplasmic cisternae. Hexagonal inclusions found in the cytoplasm of N. crassa (but not A. nidulans) and believed to arise from microbody precursors were without demonstrable cytochemical staining for microbody marker oxidases.
在沙氏葡萄糖肉汤或含有黄嘌呤或其分解代谢产物作为氮源的限定培养基中生长的粗糙脉孢菌和构巢曲霉的菌丝细胞，含有经细胞化学鉴定为微体的单膜结合细胞器。在超微结构水平上的改良Gomori方法揭示了这两种真菌细胞薄切片中假定的碱性磷酸酶活性位点。微体膜显示出电子不透明沉积物（磷酸铅），而在缺乏底物（β-甘油磷酸）或铅捕获离子的对照制剂中则不存在。在用抑制剂左旋咪唑强化的平行孵育中实现了这种酶活性的抑制。在含有有限水平无机磷酸盐的培养基中生长的细胞，在核膜和内质网池处及沿其显示出额外的碱性磷酸酶活性。在粗糙脉孢菌（而非构巢曲霉）的细胞质中发现的六边形内含物，据信源自微体前体，对于微体标记氧化酶没有可证实的细胞化学染色。
