Gainey L D, Connerton I F, Lewis E H, Turner G, Ballance D J
Department of Microbiology, University of Reading, Whiteknights, UK.
Curr Genet. 1992 Jan;21(1):43-7. doi: 10.1007/BF00318653.
The nucleotide sequences of the genes encoding the acetate-inducible glyoxylate cycle enzyme isocitrate lyase from the ascomycete fungi Aspergillus nidulans (acuD) and Neurospora crassa (acu-3) are presented. The respective A. nidulans and N. crassa genes are interrupted at identical positions by two introns and encode proteins of 538 and 543 amino acids, which have 75% identity. The predicted protein sequences do not demonstrate the C-terminal tripeptide S-K-L that has been implicated in peroxisomal targeting and found in the glyoxysomally located enzyme malate synthase from the same species. However, the protein sequences do exhibit a partial repeat which, in common with malate synthase, is located in regions that are absent from, or non-homologous with, the E. coli enzyme, which is not compartmentalized.
本文展示了编码来自子囊菌真菌构巢曲霉（acuD）和粗糙脉孢菌（acu-3）的乙酸盐诱导型乙醛酸循环酶异柠檬酸裂解酶的基因的核苷酸序列。构巢曲霉和粗糙脉孢菌各自的基因在相同位置被两个内含子打断，编码的蛋白质分别含538和543个氨基酸，二者具有75%的同一性。预测的蛋白质序列未显示出C末端三肽S-K-L，该三肽与过氧化物酶体靶向有关，且在同一物种的乙醛酸循环体定位的苹果酸合酶中存在。然而，蛋白质序列确实展现出部分重复序列，该重复序列与苹果酸合酶一样，位于大肠杆菌酶中不存在或与之非同源的区域，大肠杆菌酶不存在区室化。
