Chen Ya-Yu, Tsai Chia-Fang, Tsai Ming-Chu, Chen Wen-Kang, Hsu Yu-Wen, Lu Fung-Jou
Institute of Medicine, College of Medicine, Chung Shan Medical University, Taichung City 40201, Taiwan, China.
Department of Optometry, Da-Yeh University, Changhua 51591, Taiwan, China.
Int J Ophthalmol. 2018 Feb 18;11(2):189-195. doi: 10.18240/ijo.2018.02.02. eCollection 2018.
To investigate the anti-fibrosis effect of rosmarinic acid (RA) in pterygium epithelial cells (PECs) to determine if RA is a potent agent for treating pterygium.
The PECs (1×10 cells/mL) were treated with 100 µmol/L of RA for 1, 3 and 6h. After RA treatment, the cell viability was determined by staining with acridine orange/DAPI and analysis a NucleoCounter NC-3000. The protein expression levels of type I collagen, transforming growth factor beta-1 (TGF-β1), TGF-β type II receptor (TGF-βRII), p-Smad1/5, p-Smad2, p-Smad3, and Smad4 of the cell lysates were measured by Western blot analysis.
The cell viability of PECs was significantly decreased after RA treatment (<0.01). As the result, RA reduced the protein expression of type I collagen and TGF-β1 of PECs. Additionally, RA also inhibited TGF-β1/Smad signaling by decreasing the protein expressions of TGF-βRII, p-Smad1/5, p-Smad2, p-Smad3, and Smad4.
This study demonstrate that RA could inhibit fibrosis of PECs by down-regulating type I collagen expression and TGF-β1/Smad signaling. Therefore, RA is a potent therapeutic agent for the treatment of pterygium.
研究迷迭香酸（RA）对翼状胬肉上皮细胞（PECs）的抗纤维化作用，以确定RA是否为治疗翼状胬肉的有效药物。
将PECs（1×10个细胞/毫升）用100微摩尔/升的RA处理1、3和6小时。RA处理后，通过吖啶橙/ DAPI染色和使用NucleoCounter NC - 3000进行分析来确定细胞活力。通过蛋白质印迹分析测量细胞裂解物中I型胶原蛋白、转化生长因子β-1（TGF-β1）、TGF-β II型受体（TGF-βRII）、p-Smad1/5、p-Smad2、p-Smad3和Smad4的蛋白质表达水平。
RA处理后PECs的细胞活力显著降低（<0.01）。结果，RA降低了PECs中I型胶原蛋白和TGF-β1的蛋白质表达。此外，RA还通过降低TGF-βRII、p-Smad1/5、p-Smad2、p-Smad3和Smad4的蛋白质表达来抑制TGF-β1/Smad信号通路。
本研究表明，RA可通过下调I型胶原蛋白表达和TGF-β1/Smad信号通路来抑制PECs的纤维化。因此，RA是治疗翼状胬肉的有效治疗药物。
