Uzunoglu-Özyürek Emel, Karaaslan Hakan, Türker Sevinç Aktemur, Özçelik Bahar
Department of Endodontics, Hacettepe University Faculty of Dentistry, Ankara, Turkey.
Department of Endodontics, Bülent Ecevit University Faculty of Dentistry, Zonguldak, Turkey.
Restor Dent Endod. 2017 Dec 22;43(1):e2. doi: 10.5395/rde.2018.43.e2. eCollection 2018 Feb.
To determine the effect of size and insertion depth of irrigation needle on the amount of apical extruded debris and the amount of penetration depth of sealer using a confocal laser scanning microscope (CLSM).
Twenty maxillary premolars were assigned to 2 groups ( = 10), according to the size of needle tip, 28 G or 30 G. Buccal roots of samples were irrigated with respective needle type inserted 1 mm short of the working length (WL), while palatal roots were irrigated with respective needle type inserted 3 mm short of the WL. Prepared teeth were removed from the pre-weighed Eppendorf tubes. Canals were filled with F3 gutta-percha cone and rhodamine B dye-labeled AH 26 sealer. Teeth were transversally sectioned at 1 and 3 mm levels from the apex and observed under a CLSM. Eppendorf tubes were incubated to evaporate the irrigant and were weighed again. The difference between pre- and post-weights was calculated, and statistical evaluation was performed.
Inserting needles closer to the apex and using needles with wider diameters were associated with significantly more debris extrusion ( < 0.05). The position of needles and level of sections had statistically significant effects on sealer penetration depth ( < 0.05 for both).
Following preparation, inserting narrower needles compatible with the final apical diameter of the prepared root canal at 3 mm short of WL during final irrigation might prevent debris extrusion and improve sealer penetration in the apical third.
使用共聚焦激光扫描显微镜（CLSM）确定冲洗针的尺寸和插入深度对根尖挤出碎屑量及封闭剂渗透深度的影响。
根据针尖尺寸，将20颗上颌前磨牙分为2组（每组n = 10），分别为28G或30G。样本的颊根用各自的针型冲洗，针插入到距工作长度（WL）短1mm处，而腭根用各自的针型冲洗，针插入到距WL短3mm处。将预备好的牙齿从预先称重的微量离心管中取出。根管内填充F3牙胶尖和罗丹明B染料标记的AH 26封闭剂。将牙齿从根尖处1mm和3mm水平横向切片，并在CLSM下观察。将微量离心管孵育以蒸发冲洗液，然后再次称重。计算称重前后的差值，并进行统计学评估。
将针插入更靠近根尖的位置以及使用直径更宽的针与明显更多的碎屑挤出相关（P < 0.05）。针的位置和切片水平对封闭剂渗透深度有统计学显著影响（两者均P < 0.05）。
预备后，在最终冲洗时，使用与预备根管最终根尖直径相匹配的较窄针，在距WL短3mm处插入，可能会防止碎屑挤出并改善封闭剂在根尖三分之一处的渗透。
