Seki Yoshiyuki
Department of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University, Hyogo, Japan.
Front Cell Dev Biol. 2018 Feb 13;6:12. doi: 10.3389/fcell.2018.00012. eCollection 2018.
PR-domain containing protein 14 (PRDM14) is a site-specific DNA-binding protein and is required for establishment of pluripotency in embryonic stem cells (ESCs) and primordial germ cells (PGCs) in mice. DNA methylation status is regulated by the balance between methylation and passive/active demethylation, and global DNA hypomethylation is closely associated with cellular pluripotency and totipotency. PRDM14 ensures hypomethylation in mouse ESCs and PGCs through two distinct layers, transcriptional repression of the DNA methyltransferases and active demethylation by recruitment of TET proteins. However, the function of PRDM14 remains unclear in other species including humans. Hence, here we focus on the unique characteristics of mouse PRDM14 in the epigenetic regulation of pluripotent cells and primordial germ cells. In addition, we discuss the expression regulation and function of PRDM14 in other species compared with those in mice.
含PR结构域蛋白14（PRDM14）是一种位点特异性DNA结合蛋白，在小鼠胚胎干细胞（ESC）和原始生殖细胞（PGC）多能性建立过程中发挥作用。DNA甲基化状态受甲基化与被动/主动去甲基化之间平衡的调节，全基因组DNA低甲基化与细胞多能性和全能性密切相关。PRDM14通过两个不同层面确保小鼠ESC和PGC中的低甲基化，即对DNA甲基转移酶进行转录抑制以及通过募集TET蛋白进行主动去甲基化。然而，PRDM14在包括人类在内的其他物种中的功能尚不清楚。因此，在这里我们聚焦于小鼠PRDM14在多能细胞和原始生殖细胞表观遗传调控中的独特特征。此外，我们还讨论了与小鼠相比，PRDM14在其他物种中的表达调控及功能。
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