Mouahid Gabriel, Rognon Anne, de Carvalho Augusto Ronaldo, Driguez Patrick, Geyer Kathy, Karinshak Shannon, Luviano Nelia, Mann Victoria, Quack Thomas, Rawlinson Kate, Wendt George, Grunau Christoph, Moné Hélène
Laboratoire Interactions Hôtes-Pathogènes-Environnements (IHPE), UMR 5244 CNRS/UPVD/IFREMER/UM, University of Perpignan Via Domitia, 58 Avenue Paul Alduy, Bât R, F-66860 Perpignan Cedex, France.
Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, UK.
Wellcome Open Res. 2018 Jan 9;3:3. doi: 10.12688/wellcomeopenres.13488.1. eCollection 2018.
Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and transferred from one snail to another. This procedure provides a technical basis for different types of modifications, such as those used in functional genomics studies.
血吸虫病是一种重要的寄生虫病，全球约有2亿人受其影响。病原体为不同种类。血吸虫具有复杂的生命周期，淡水螺为中间宿主。感染后，子孢子在螺宿主内发育并产生寄生于人体的幼虫。我们在此提供一份详细的分步视频指南，有英语、法语、西班牙语和葡萄牙语版本，展示了如何操作这些子孢子并将其从一只螺转移到另一只螺。该程序为不同类型的改造提供了技术基础，比如用于功能基因组学研究的改造。
